A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
文献类型：期刊论文
| 作者 | Feng, Lifang1; Liu, Xiaohan1; Cao, Hongjuan1; Qin, Limei1; Hou, Wentao2; Wu, Lin1 |
| 刊名 | JOURNAL OF ULTRASOUND IN MEDICINE
 |
| 出版日期 | 2019 |
| 卷号 | 38期号:1页码:191-202 |
| 关键词 | bone formation frequency low-intensity pulsed ultrasound osteogenesis porous Ti64 scaffolds |
| ISSN号 | 0278-4297 |
| DOI | 10.1002/jum.14683 |
| 通讯作者 | Wu, Lin(wulin13@163.com) |
| 英文摘要 | Objectives Low-intensity pulsed ultrasound (LIPUS) combined with porous scaffolds can be used as a new therapy to treat bone defect repair. The aim of this study was to evaluate the effects of 1 and 3.2 MHz LIPUS on osteogenesis on porous Ti64 alloy scaffolds for both in vitro and in vivo studies. Methods Scaffolds were randomly divided into the high-frequency ultrasound group, low-frequency ultrasound group, and control group. Mouse pre-osteoblast cells were cultured with porous Ti-6Al-4V scaffolds in vitro to evaluate cell proliferation and differentiation. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated by observing the micro-computed tomography (micro-CT), toluidine blue staining, and von Kossa staining. Results The results revealed no significant difference in the cell counting kit-8 values between the ultrasound groups and control groups (P > .05). Compared with the control group, ultrasound promoted alkaline phosphatase activity and osteocalcin levels of the cells on the scaffolds (P < .05), but there was no significant difference between the two frequencies. In addition, histomorphologic analyses revealed that the volume and amount of new bone formation increased and that bone maturity improved in the ultrasound groups compared with the control group, but no significant difference was noted between the two frequencies. Conclusions Under the present experimental conditions, LIPUS promoted osteoblast differentiation and promoted bone maturity on porous Ti64 scaffolds. No significant differences were noted between the two frequencies. |
| 资助项目 | National High Technology Research & Development Program (863 Program) of China[2015AA033702] ; Nature Science Foundation of Liaoning Province, China[2014021008] |
| WOS研究方向 | Acoustics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000453869000022 |
| 出版者 | WILEY |
| 资助机构 | National High Technology Research & Development Program (863 Program) of China ; Nature Science Foundation of Liaoning Province, China |
| 源URL | [http://ir.imr.ac.cn/handle/321006/130899]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Wu, Lin |
| 作者单位 | 1.China Med Univ, Sch Stomatol, Dept Prosthodont, 117 Nanjing North St, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,et al. A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study[J]. JOURNAL OF ULTRASOUND IN MEDICINE,2019,38(1):191-202. |
| APA | Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,Qin, Limei,Hou, Wentao,&Wu, Lin.(2019).A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study.JOURNAL OF ULTRASOUND IN MEDICINE,38(1),191-202. |
| MLA | Feng, Lifang,et al."A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study".JOURNAL OF ULTRASOUND IN MEDICINE 38.1(2019):191-202. |
入库方式： OAI收割
来源：金属研究所
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