Light-sheet microscopy in the near-infrared II window
文献类型：期刊论文
| 作者 | Wang, Feifei1; Wan, Hao1; Ma, Zhuoran1; Zhong, Yeteng1; Sun, Qinchao1; Tian, Ye1; Qu, Liangqiong2,8; Du, Haotian1; Zhang, Mingxi9; Li, Lulin3 |
| 刊名 | NATURE METHODS
 |
| 出版日期 | 2019-06-01 |
| 卷号 | 16期号:6页码:545-+ |
| ISSN号 | 1548-7091 |
| DOI | 10.1038/s41592-019-0398-7 |
| 通讯作者 | Dai, Hongjie(hdai@stanford.edu) |
| 英文摘要 | Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site. |
| 资助项目 | National Institutes of Health[DP1-NS-105737] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000469455200024 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | National Institutes of Health |
| 源URL | [http://ir.imr.ac.cn/handle/321006/133742]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Dai, Hongjie |
| 作者单位 | 1.Stanford Univ, Dept Chem & BioX, Stanford, CA 94305 USA 2.Univ N Carolina, Dept Radiol, Chapel Hill, NC USA 3.VA Palo Alto Hlth Care Syst, Palo Alto Vet Inst Res, Palo Alto, CA USA 4.Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA 5.City Univ Hong Kong, Dept Mech Engn, Hong Kong, Peoples R China 6.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang, Liaoning, Peoples R China 7.Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA 8.Univ N Carolina, BRIC, Chapel Hill, NC USA 9.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan, Hubei, Peoples R China 10.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Feifei,Wan, Hao,Ma, Zhuoran,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-+. |
| APA | Wang, Feifei.,Wan, Hao.,Ma, Zhuoran.,Zhong, Yeteng.,Sun, Qinchao.,...&Dai, Hongjie.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-+. |
| MLA | Wang, Feifei,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-+. |
入库方式： OAI收割
来源：金属研究所
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