Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation
文献类型：期刊论文
| 作者 | Yuan, Yonghui2,3; Jin, Shujing4; Qi, Xun2; Chen, Xudong1,5; Zhang, Wei1,5; Yang, Ke4; Zhong, Hongshan2 |
| 刊名 | JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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| 出版日期 | 2019-11-01 |
| 卷号 | 35期号:11页码:2727-2733 |
| 关键词 | Osteogenesis Cu addition Stainless steel Cell signaling pathway Bone |
| ISSN号 | 1005-0302 |
| DOI | 10.1016/j.jmst.2019.04.028 |
| 通讯作者 | Yang, Ke(kyang@imr.ac.cn) ; Zhong, Hongshan(hszhong@cmu.edu.cn) |
| 英文摘要 | As a metallic orthopedic implant, 316L stainless steel (316L SS) is used extensively for its good resistance to corrosion and mechanical properties. However, it takes a long time to achieve osseointegration between 316 L SS and adjacent tissues due to its bio-inert characteristic. Hence, the aim is to improve the bio-adaption of 316 L SS. A good approach is to add elements to materials to improve their osteogenic capabilities by the appropriate release of ions. Hence copper-containing 316 L stainless steel (316L-Cu SS) was investigated in this work, where Cu is an essential trace element that can stimulates osteogenesis. It was found that 316L-Cu SS was bio-safe and did not affect the proliferation of co-cultured osteoblasts in comparison with 316 L SS. It increased cell apoptosis on day 1 but inhibited it on day 3, which cooperates with new bone formation processes. Osteoblasts extend themselves more quickly and in a better manner on the surface of 316L-Cu SS, wheneven more pseudopodia are present. Furthermore, the gene expression of alkaline phosphatase, collagen I and runt-related transcription factor 2 (Runx2) in osteoblasts cultured with 316L-Cu SS was significantly enhanced. Runx2 protein expression increased, and osteogenesis was stimulated by 316L-Cu SS via an Akt cell signaling pathway. In conclusion, 316L-Cu SS stimulates osteogenesis through activation of the Akt cell signaling pathway and the upregulation of Runx2. Thus, 316L-Cu SS is a promising material that may be used in surgical implants to stimulate osteogenesis. (C) 2019 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology. |
| 资助项目 | National Key Research and Development Program of China[2018YFC1106600] ; National Natural Science Foundation[81571778] ; National Natural Science Foundation[51631009] ; Science and Technology Plan of Shenyang[17-230-9-42] ; Science and Technology Plan of Shenyang[18-014-4-28] |
| WOS研究方向 | Materials Science ; Metallurgy & Metallurgical Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000497965900034 |
| 出版者 | JOURNAL MATER SCI TECHNOL |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation ; Science and Technology Plan of Shenyang |
| 源URL | [http://ir.imr.ac.cn/handle/321006/136202]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Yang, Ke; Zhong, Hongshan |
| 作者单位 | 1.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China 2.China Med Univ, Affiliated Hosp 1, Dept Radiol, Key Lab Diagnost Imaging & Intervent Radiol Liaon, Shenyang 110001, Liaoning, Peoples R China 3.China Med Univ, Liaoning Canc Hosp & Inst, Canc Hosp, Res & Acad Dept, Shenyang 110042, Liaoning, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 5.Shenzhen Intervent Med Engn Res & Dev Ctr, Shenzhen 518020, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yonghui,Jin, Shujing,Qi, Xun,et al. Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2019,35(11):2727-2733. |
| APA | Yuan, Yonghui.,Jin, Shujing.,Qi, Xun.,Chen, Xudong.,Zhang, Wei.,...&Zhong, Hongshan.(2019).Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation.JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,35(11),2727-2733. |
| MLA | Yuan, Yonghui,et al."Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation".JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 35.11(2019):2727-2733. |
入库方式： OAI收割
来源：金属研究所
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