Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells
文献类型：期刊论文
| 作者 | Zhang, Ji; Ren, Ling; Yang, Ke |
| 刊名 | ACTA METALLURGICA SINICA-ENGLISH LETTERS
 |
| 出版日期 | 2020-11-11 |
| 页码 | 7 |
| 关键词 | Cytotoxicity Ti– 6Al– 4V– 5Cu alloy Fabrication Biocompatibility |
| ISSN号 | 1006-7191 |
| DOI | 10.1007/s40195-020-01158-1 |
| 通讯作者 | Yang, Ke(kyang@imr.ac.cn) |
| 英文摘要 | The aim of this work was to study the cytotoxicity of Ti-6Al-4V-5Cu (TC4-Cu) alloy for dental applications and evaluate the cell viability of different fabricated TC4-Cu alloys in contact with MC3T3-E1 cells in vitro. Ti-6Al-4V (TC4) alloy was used as a negative control to evaluate the cytotoxicity level of TC4-Cu alloy so as to provide basic support for the dental clinical application. Control group TC4 and experimental group TC4-Cu with different fabrications were incubated in the cell culture medium. The absorbance value of mouse osteoblast MC3T3-E1 cells was detected by CCK-8 assay after 1, 3 and 7 days, respectively. The relative proliferation rate of cells was calculated, and then the toxicity level was valued for each group. Cell morphology on the surface was also studied by observing the cytoskeleton through F-actin filament staining. The experimental results showed that the absorbance values for the experimental and the negative control groups were not same at different time points. Compared with different fabricated TC4-Cu alloys, the annealing-TC4-Cu alloy showed much better biocompatibility. The mouse osteoblast MC3T3-E1 cells cultured with annealing-TC4-Cu, rolling-TC4-Cu and solution + aging-TC4-Cu have no toxic effects, and these alloys could promote the proliferation of mouse osteoblasts. |
| 资助项目 | National Natural Science Foundation of China[51631009] |
| WOS研究方向 | Metallurgy & Metallurgical Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000588588000003 |
| 出版者 | CHINESE ACAD SCIENCES, INST METAL RESEARCH |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.imr.ac.cn/handle/321006/141315]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Yang, Ke |
| 作者单位 | Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ji,Ren, Ling,Yang, Ke. Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells[J]. ACTA METALLURGICA SINICA-ENGLISH LETTERS,2020:7. |
| APA | Zhang, Ji,Ren, Ling,&Yang, Ke.(2020).Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells.ACTA METALLURGICA SINICA-ENGLISH LETTERS,7. |
| MLA | Zhang, Ji,et al."Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells".ACTA METALLURGICA SINICA-ENGLISH LETTERS (2020):7. |
入库方式： OAI收割
来源：金属研究所
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