Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells
文献类型：期刊论文
| 作者 | Zou Defeng2; Chen Yi1; Han Yaxin2; Lv Chen2; Tu Guanjun2 |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:12页码:1241-1248 |
| 关键词 | SPINAL-CORD-INJURY NERVOUS-SYSTEM MIR-124 FATE NEUROGENESIS EXPRESSION REGULATORS PROTECTS COREST REST nerve regeneration microRNA-124 lentivirus overexpression bone marrow-derived mesenchymal stem cells neural stem cells spinal cord injury neurogenesis GeneChip motor function NSFC grant neural regeneration |
| ISSN号 | 1673-5374 |
| 其他题名 | Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells |
| 英文摘要 | microRNAs (miRNAs) play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 (miR-124) overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs in bone marrow-derived mesenchymal stem cells, neural stem cells and neurons. miR-124 expression was substantially reduced in bone marrow-derived mesenchymal stem cells compared with the other cell types. We constructed a lentiviral vector overexpressing miR-124 and transfected it into bone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markers beta-III tubulin and microtubule-associated protein-2 were significantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These results suggest that miR-124 plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells into neurons. Our findings should facilitate the development of novel strategies for enhancing the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for spinal cord injury. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5185878 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/141748]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.浙江大学 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Zou Defeng,Chen Yi,Han Yaxin,et al. Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells[J]. NEURAL REGENERATION RESEARCH,2014,9(12):1241-1248. |
| APA | Zou Defeng,Chen Yi,Han Yaxin,Lv Chen,&Tu Guanjun.(2014).Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells.NEURAL REGENERATION RESEARCH,9(12),1241-1248. |
| MLA | Zou Defeng,et al."Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells".NEURAL REGENERATION RESEARCH 9.12(2014):1241-1248. |
入库方式： OAI收割
来源：金属研究所
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