Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
文献类型：期刊论文
| 作者 | Wang QiuYue1; Li JianHua1; Zhang XiChen1; Liu ChengWu3; Cao LiLi1; Ren KeYan2; Gong PengTao1; Cai YaNan1 |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2009 |
| 卷号 | 52期号:3页码:278-283 |
| 关键词 | IMMUNE-RESPONSES ANTIGEN DELIVERY EIMERIA-TENELLA VACCINE VECTORS BCG COCCIDIOSIS PROTECTION CHALLENGE IMMUNIZATION TOXOPLASMA coccidiosis rBCG EGFP rhomboid laser confocal microscopy real-time RT-PCR |
| ISSN号 | 1006-9305 |
| 其他题名 | Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens |
| 英文摘要 | Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3590941 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/141859]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.吉林大学 2.中山大学 3.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Wang QiuYue,Li JianHua,Zhang XiChen,et al. Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2009,52(3):278-283. |
| APA | Wang QiuYue.,Li JianHua.,Zhang XiChen.,Liu ChengWu.,Cao LiLi.,...&Cai YaNan.(2009).Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,52(3),278-283. |
| MLA | Wang QiuYue,et al."Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 52.3(2009):278-283. |
入库方式： OAI收割
来源：金属研究所
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