Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase
文献类型：期刊论文
| 作者 | Zhu YanWen1; Liu Ling1; Sun WeiJie1; Yan TingCai1; Tan DeHong1; Zhang Yao2; Bai Bing1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018 |
| 卷号 | 46期号:7页码:1145-1151 |
| 关键词 | FRESH GARLIC SCALES H2S EXPLORATION OXIDATION Hydrogen sulfide L-Lactic dehydrogenase Cysteine thiols S-Sulfuration |
| ISSN号 | 0253-3820 |
| 其他题名 | Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase |
| 英文摘要 | Molecular mechanisms whereby H2 S influences its targets have been of intriguing interest. In this work, L-lactic dehydrogenase (L-LDH) was used as the protein target, and three kinds of H2 S-donor reagents ( NaHS, Na2S, and polysulfide) were chosen. The interactions of these H2 S-donor reagents with L-LDH were disclosed by molecular fluorescent assays for real-time monitoring of L-LDH activity. The results of the SDSPAGE showed that H2 S might not interact with L-LDH to form disulfide/trisulfide bonding. Circular dichroism spectra assays revealed that H2 S reagents could be likely to react with cysteine thiols to yield sulfurated thiol ( SSH) derivatives in L-LDH, and sulfur-containing PS ( polysulfide) was a stronger protein S-sulfurating agent than the other two sulfides. Matrix assisted laser desorptionionization time-of-flight tandem mass spectrometry ( MALDI-TOF-MS/MS) study showed partial S-sulfuration of the active cysteine sites existed in L-LDH. In conclusion, H25 exerts its biological effects as a gasotransmitter through its reactions with cysteine thiols in proteins by S-sulfuration. |
| 资助项目 | [National Natural Science Foundation of China] ; [National Key Research Projects of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6284574 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/142162]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.沈阳师范大学 |
| 推荐引用方式 GB/T 7714 | Zhu YanWen,Liu Ling,Sun WeiJie,et al. Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(7):1145-1151. |
| APA | Zhu YanWen.,Liu Ling.,Sun WeiJie.,Yan TingCai.,Tan DeHong.,...&Bai Bing.(2018).Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(7),1145-1151. |
| MLA | Zhu YanWen,et al."Investigation of Interaction Mechanism Between Signaling Molecule Hydrogen Sulfide and L-Lactic Dehydrogenase".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.7(2018):1145-1151. |
入库方式： OAI收割
来源：金属研究所
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