Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil
文献类型：期刊论文
| 作者 | Liu Lingzhi; Qin Sijun; Lu Deguo; Wang Bingying; Yang Zeyuan |
| 刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| 出版日期 | 2014 |
| 卷号 | 13期号:2页码:415-425 |
| 关键词 | GRADIENT GEL-ELECTROPHORESIS CONIFEROUS FOREST SOILS 16S RIBOSOMAL-RNA NITRIFYING BACTERIA LOW PH PCR DIVERSITY ACID POPULATIONS ARCHAEA apple orchard soil ammonia-oxidizing bacteria potential nitrification community structure PCR-denaturing gradient gel electrophoresis |
| ISSN号 | 2095-3119 |
| 其他题名 | Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil |
| 英文摘要 | In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil, The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the beta-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus, Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics. |
| 资助项目 | [National Natural Science Foundation of China] ; [Postdoctoral Science Foundation of China] ; [China Agricultural Research System] ; [Shenyang Municipal Science and Technology Research Projects, China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5048474 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/142393]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Liu Lingzhi,Qin Sijun,Lu Deguo,et al. Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(2):415-425. |
| APA | Liu Lingzhi,Qin Sijun,Lu Deguo,Wang Bingying,&Yang Zeyuan.(2014).Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil.JOURNAL OF INTEGRATIVE AGRICULTURE,13(2),415-425. |
| MLA | Liu Lingzhi,et al."Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil".JOURNAL OF INTEGRATIVE AGRICULTURE 13.2(2014):415-425. |
入库方式： OAI收割
来源：金属研究所
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