Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
文献类型：期刊论文
| 作者 | Zhang Ning; Jiang Jing; Yang Yanli; Wang Zhihe |
| 刊名 | JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
 |
| 出版日期 | 2015 |
| 卷号 | 16期号:10页码:845-856 |
| 关键词 | CELL-WALL INVERTASE APOPLASTIC INVERTASE VACUOLAR INVERTASE PLANT DEVELOPMENT CARBOHYDRATE-METABOLISM EXTRACELLULAR INVERTASE SOLANUM-LYCOPERSICON STRUCTURAL INSIGHTS ECTOPIC EXPRESSION CARBON METABOLISM Invertase inhibitor Fruit development Transient transformation system Solanum lycopersicum Overexpression |
| ISSN号 | 1673-1581 |
| 其他题名 | Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit * |
| 英文摘要 | In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato. |
| 资助项目 | [National Natural Science Foundation of China] ; [State Key Laboratory of Plant Physiology and Biochemistry Open Project of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5544667 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/142721]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Ning,Jiang Jing,Yang Yanli,et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2015,16(10):845-856. |
| APA | Zhang Ning,Jiang Jing,Yang Yanli,&Wang Zhihe.(2015).Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,16(10),845-856. |
| MLA | Zhang Ning,et al."Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 16.10(2015):845-856. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。