Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts
文献类型：期刊论文
| 作者 | Zhang JinChao1; Liu DanDan1; Sun Jing1; Zhang DaWei2; Shen ShiGang1; Yang MengSu3 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2009 |
| 卷号 | 54期号:1页码:66-71 |
| 关键词 | OSTEOCLAST-LIKE CELLS IN-VITRO SUPPORT DIFFERENTIATION PROLIFERATION OSTEOPOROSIS RESORPTION CULTURES PROTEIN GROWTH IONS rare earth ion bone marrow stromal cells osteogenic differentiation adipogenic differentiation adipocytic trans-differentiation |
| ISSN号 | 1001-6538 |
| 其他题名 | Effect of Dy~(3+) on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts |
| 英文摘要 | A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase ( ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the effect of Dy3+ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation of mouse primary osteoblasts (OBs). The results showed that Dy3+ had no effect on BMSC proliferation at concentrations of 1 x 10(-8) and 1 x 10(-5) mol/L, but inhibited BMSC proliferation at other concentrations. Dy3+ had no effect on OB proliferation at concentrations of 1 x 10(-10) and 1 x 10(-9) mol/L, but inhibited OB proliferation at other concentrations. Dy3+ had no effect on the osteogenic differentiation of BMSCs at concentrations of 1 x 10(-9) and 1 x 10(-7) mol/ L, and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day. The osteogenic differentiation of BMSCs was inhibited by Dy3+ at concentration of 1 x 10(-5) mol/ L at the 14th day, but promoted osteogenic differentiation of BMSCs at concentrations of 1 x 10(-9), 1 x 10(-8), 1 x 10(-7) and 1 x 10(-6) mol/ L with the maximal effect at concentration of 10(-6) mol/ L. Dy3+ promoted mineralized function of BMSCs at any concentration. Dy3+ had no effect on adipogenic differentiation of BMSCs at concentration of 1 x 10(-7) mol/ L, but inhibited adipogenic differentiation of BMSCs at other concentrations. Dy3+ inhibited adipocytic trans-differentiation of OBs at any concentration, suggesting that Dy3+ had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization of OBs. These results may be valuable for better understanding the mechanism of the effect of Dy3+ on pathogenesis of osteoporosis. |
| 资助项目 | [Ministry of Education of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3565991 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/143144]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.河北大学 2.中国科学院金属研究所 3.City University Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang JinChao,Liu DanDan,Sun Jing,et al. Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts[J]. CHINESE SCIENCE BULLETIN,2009,54(1):66-71. |
| APA | Zhang JinChao,Liu DanDan,Sun Jing,Zhang DaWei,Shen ShiGang,&Yang MengSu.(2009).Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts.CHINESE SCIENCE BULLETIN,54(1),66-71. |
| MLA | Zhang JinChao,et al."Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts".CHINESE SCIENCE BULLETIN 54.1(2009):66-71. |
入库方式： OAI收割
来源：金属研究所
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