Application of phage-displayed single chain antibodies in western blot
文献类型：期刊论文
| 作者 | Ding YL1; Liu MY1; Han W1; Yang SL1; Liu H1; Gong Y1 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:3页码:205-209 |
| 关键词 | B-LYMPHOCYTE STIMULATOR FUSION PHAGE TUMOR-CELLS LIBRARY ANTIGENS PROTEIN IDENTIFICATION SELECTION PEPTIDE BLYS phage-display anitbody Western blot |
| ISSN号 | 1672-9145 |
| 其他题名 | Application of Phage-displayed Single Chain Antibodies in Western Blot |
| 英文摘要 | A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1958580 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/143790]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Ding YL,Liu MY,Han W,et al. Application of phage-displayed single chain antibodies in western blot[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(3):205-209. |
| APA | Ding YL,Liu MY,Han W,Yang SL,Liu H,&Gong Y.(2005).Application of phage-displayed single chain antibodies in western blot.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(3),205-209. |
| MLA | Ding YL,et al."Application of phage-displayed single chain antibodies in western blot".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.3(2005):205-209. |
入库方式： OAI收割
来源：金属研究所
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