Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
文献类型：期刊论文
| 作者 | Wang M2; Li QR2; Di Fiore S2; Fischer R2 |
| 刊名 | ACTA BOTANICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 44期号:3页码:314-317 |
| 关键词 | CATHARANTHUS-ROSEUS ALKALOIDS PROTEINS ER tryptophan decarboxylase alkaloids biosynthetic pathway Nicotiana tabacum subcellular compartment |
| ISSN号 | 0577-7496 |
| 其他题名 | Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant |
| 英文摘要 | The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1365022 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/145596]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.中国科学院 3.Rhein Westfal TH Aachen |
| 推荐引用方式 GB/T 7714 | Wang M,Li QR,Di Fiore S,et al. Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant[J]. ACTA BOTANICA SINICA,2002,44(3):314-317. |
| APA | Wang M,Li QR,Di Fiore S,&Fischer R.(2002).Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant.ACTA BOTANICA SINICA,44(3),314-317. |
| MLA | Wang M,et al."Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant".ACTA BOTANICA SINICA 44.3(2002):314-317. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。