LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
文献类型：期刊论文
| 作者 | Shi Meiyun1; Yin Lei1; Cai Lanlan1; Wang Can1; Liu Xidong1; Zhao Sen1; Sun Yantong1; Fawcett J Paul3; Zhao Limei4; Yang Yan1 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2014 |
| 卷号 | 30期号:6页码:900-904 |
| 关键词 | HEALTHY CHINESE VOLUNTEERS DISODIUM SINGLE INJECTION Cefotetan Liquid chromatography-tandem mass spectrometry(LC-MS/MS) Pharmacokinetics Human plasma |
| ISSN号 | 1005-9040 |
| 其他题名 | LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study |
| 英文摘要 | A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C-8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3 -> 460.2(quantifier) and m/z 576.3 -> 432.2(qualifier) and for IS at m/z 264.1 -> 58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1 -> 100 mu g/mL for 20 mu L of human plasma only with intra-and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of +/- 1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6). |
| 资助项目 | [National Natural Science Foundation of China] ; [Preject of China Equipment and Education Resources System(CERS)] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5301737 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/145675]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.吉林大学 2.中山大学 3.University Otago, Sch Pharm, Dunedin 9054, New Zealand 4.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Shi Meiyun,Yin Lei,Cai Lanlan,et al. LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2014,30(6):900-904. |
| APA | Shi Meiyun.,Yin Lei.,Cai Lanlan.,Wang Can.,Liu Xidong.,...&Gu Jingkai.(2014).LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,30(6),900-904. |
| MLA | Shi Meiyun,et al."LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 30.6(2014):900-904. |
入库方式： OAI收割
来源：金属研究所
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