Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector
文献类型：期刊论文
| 作者 | Zhang Yi2; Zhang Xue2; Xia Huanzhang3; Xue Yuegui1; Wang Jianyang2; Tian Baozhong2; Wei Zhenguo2; Lu Changde2 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:6页码:533-538 |
| 关键词 | EXPRESSION SYSTEM EGFP promoter activity A3 IE-1 silkworm tissue rAcNPV |
| ISSN号 | 1672-9145 |
| 其他题名 | Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector |
| 英文摘要 | Cassettes harboring luciferase reporter driven by Bombyx mori cytoplasmic actin gene promoter (A3) (671 bp) and B. mori nuclear polyhedrosis virus immediate-early promoter (IE-1) (580 bp) were transferred to the bacmid Ac Delta EGT to generate the recombinant Autographa californica nuclear polyhedrosis viruses, AcNPVA3Luc and AcNPVIELuc, respectively. Recombinant baculoviruses were injected into the hemocoele of newly ecdysed 5th instar larvae. The activities of the A3 and IE-1 promoters in various tissues were measured by luciferase activity assay and normalized by the copy number of recombinant virus. Results showed that the activity of the A3 promoter was approximately 10-fold higher than the IE-1 promoter. The promoter activities of A3 and IE-1 were highest in the silk gland, followed by fat body, middle gut, Malpighian tubule, and hemocyte. In silk gland, activity of the two promoters was highest in posterior silk gland, followed by middle and anterior silk glands. The difference in promoter activities reflects the growth speed of tissue in silkworm larvae. The activity of the A3 promoter remained unchanged and was not inhibited significantly by viral factors at least 3-4 d post injection of rAcNPV. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3301268 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/145893]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.广西师范大学 2.中国科学院 3.中国科学院金属研究所 4.苏州大学 |
| 推荐引用方式 GB/T 7714 | Zhang Yi,Zhang Xue,Xia Huanzhang,et al. Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(6):533-538. |
| APA | Zhang Yi.,Zhang Xue.,Xia Huanzhang.,Xue Yuegui.,Wang Jianyang.,...&Lu Changde.(2008).Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(6),533-538. |
| MLA | Zhang Yi,et al."Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.6(2008):533-538. |
入库方式： OAI收割
来源：金属研究所
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