Stem cell properties and neural differentiation of sheep amniotic epithelial cells
文献类型：期刊论文
| 作者 | Zhu Xuemin1; Wang Xiumei1; Cao Guifang1; Liu Fengjun2; Yang Yinfeng1; Li Xiaonan3; Zhang Yuling2; Mi Yan1; Liu Junping1; Zhang Lingli1 |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:13页码:1210-1219 |
| 关键词 | PRIMORDIAL GERM-CELLS IN-VITRO PARKINSONS-DISEASE HUMAN BLASTOCYSTS MOUSE EMBRYOS IPS CELL TRANSPLANTATION LINES NEURONS CULTURE neural regeneration stem cells sheep amniotic epithelial cells isolation and culture stem cell characteristics differentiation differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper neuroregeneration |
| ISSN号 | 1673-5374 |
| 其他题名 | Stem cell properties and neural differentiation of sheep amniotic epithelial cells ☆ |
| 英文摘要 | This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed beta-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing beta-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. |
| 资助项目 | [National High-Tech Research and Development Program of China (863 Program)] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4862026 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/145938]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Inner Mongolia Agr University, Coll Vet Med, Hohhot 010018, Inner Mongolia, Peoples R China 2.河南大学 3.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Zhu Xuemin,Wang Xiumei,Cao Guifang,et al. Stem cell properties and neural differentiation of sheep amniotic epithelial cells[J]. NEURAL REGENERATION RESEARCH,2013,8(13):1210-1219. |
| APA | Zhu Xuemin.,Wang Xiumei.,Cao Guifang.,Liu Fengjun.,Yang Yinfeng.,...&Zhang Lingli.(2013).Stem cell properties and neural differentiation of sheep amniotic epithelial cells.NEURAL REGENERATION RESEARCH,8(13),1210-1219. |
| MLA | Zhu Xuemin,et al."Stem cell properties and neural differentiation of sheep amniotic epithelial cells".NEURAL REGENERATION RESEARCH 8.13(2013):1210-1219. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。