Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis
文献类型：期刊论文
| 作者 | Li Hui1; Lang KunLing1; Fu HaiBin2; Shen ChangPeng1; Wan FangHao1; Chu Dong1 |
| 刊名 | INSECT SCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 22期号:6页码:761-767 |
| 关键词 | TINSLEY HEMIPTERA PSEUDOCOCCIDAE RNA INTERFERENCE HYPERTREHALOSEMIC HORMONE GENE COCKROACH PEST expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis |
| ISSN号 | 1672-9609 |
| 其他题名 | Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis |
| 英文摘要 | The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. |
| 资助项目 | [National Science and Technology Support Program] ; [Science and Technology Development Planning Program of Qingdao] ; [Taishan Mountain Scholar Constructive Engineering Foundation of Shandong] ; [Shandong Modern Agricultural Technology & Industry System] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5582479 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/147613]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.黑龙江省环境保护科学研究院 2.中国科学院金属研究所 3.Chinese Acad Agr Sci, Institute Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China Institute Plant Protect State Key Lab Biol Plant Dis & Insect Pests |
| 推荐引用方式 GB/T 7714 | Li Hui,Lang KunLing,Fu HaiBin,et al. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis[J]. INSECT SCIENCE,2015,22(6):761-767. |
| APA | Li Hui,Lang KunLing,Fu HaiBin,Shen ChangPeng,Wan FangHao,&Chu Dong.(2015).Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis.INSECT SCIENCE,22(6),761-767. |
| MLA | Li Hui,et al."Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis".INSECT SCIENCE 22.6(2015):761-767. |
入库方式： OAI收割
来源：金属研究所
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