Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells
文献类型：期刊论文
| 作者 | Yue Dan; Wang Yong; Ma Ping; Li YinYan; Chen Hong; Wang Ping; Ren ChangShan |
| 刊名 | ASIAN JOURNAL OF ANDROLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 12期号:3页码:381-389 |
| 关键词 | HEPATOCYTE GROWTH-FACTOR RECEPTOR C-MET NUDE-MICE HGF ANGIOGENESIS METASTASIS ANTAGONIST BENIGN EXPRESSION CARCINOMA hepatocyte growth factor human prostate cancer NK4 DU145 cells |
| ISSN号 | 1008-682X |
| 其他题名 | Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells |
| 英文摘要 | We investigated the ability of NK4, an antagonist of human hepatocyte growth factor (HGF), to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro. In vivo, we subcutaneously implanted DU145 cells, mock-transfected clone (DU145/empty vector) cells and NK4-transfected clone (DU145/NK4) cells into nude mice, and then evaluated tumor growth, cell proliferation and cell apoptosis in vivo. We found that DU145/NK4 cells expressed NK4 protein. In the in vitro study, autocrine NK4 attenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 (ERK1). and protein kinase B 1/2 (Akt1/2). Histological examination revealed that autocrine NK4 inhibited proliferation and accelerated apoptosis of prostate cancer cells. These results show that genetic modification of DU145 cells with NK4 cDNA yields a significant effect on their proliferation, migration, invasion and apoptosis. Molecular targeting of HGF/c-Met by NK4 could be applied as a novel therapeutic approach to prostate cancer. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3891332 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/148565]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Yue Dan,Wang Yong,Ma Ping,et al. Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(3):381-389. |
| APA | Yue Dan.,Wang Yong.,Ma Ping.,Li YinYan.,Chen Hong.,...&Ren ChangShan.(2010).Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells.ASIAN JOURNAL OF ANDROLOGY,12(3),381-389. |
| MLA | Yue Dan,et al."Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells".ASIAN JOURNAL OF ANDROLOGY 12.3(2010):381-389. |
入库方式： OAI收割
来源：金属研究所
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