Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement
文献类型：期刊论文
| 作者 | Zhang Lili1; Wu Zhijie1; Song Yuchao2 |
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:7页码:2011-2013 |
| 关键词 | NITROUS-OXIDE NITRIFICATION DINITROGEN SOILS N-15 Mass spectrometery Denitrifying enzyme activity 3,4-dimethylpyrazole phosphate |
| ISSN号 | 1000-0593 |
| 其他题名 | Mass spectrometery assay for denitrifying enzyme activity measurement |
| 英文摘要 | Nitrification inhibitors exert inhibition function in soil nitrification process (NF4+-N to NO3--N) and are widely applied in order to improve N fertilizer use efficiency. Before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (DEA) is an effective indicator to show this process. In the present paper, a mass spectrometery(MS) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(DMPP) incubation system. The results showed that the method measured the concentration of N2O in the incubation system accurately and the concentration measured by MS had marked correlation with that measured by gas chromatogram(GC) (MSN2O = -0.45 + 1.03GC(N2O), R-2 = 0.995). At the same time, enrichment of (N2O)-N-15 and N-15(2) was measured to discriminate the nitrate reductase and N2O reductase. Differed with traditional method, the enzymatic inhibitor-acetylene was not appended. The results showed that DMPP had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by DMPP. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3913888 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/148901]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Lili,Wu Zhijie,Song Yuchao. Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(7):2011-2013. |
| APA | Zhang Lili,Wu Zhijie,&Song Yuchao.(2010).Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(7),2011-2013. |
| MLA | Zhang Lili,et al."Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.7(2010):2011-2013. |
入库方式： OAI收割
来源：金属研究所
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