Adenosine triphosphate-sensitive potassium channel opener protects PC12 cells against hypoxia-induced apoptosis through PI3K/Akt and Bcl-2 signaling pathways
文献类型：期刊论文
| 作者 | Zhang Hong; Jia Chunhong; Zhao Danyang; Lu Yang; Wang Runling; Li Jia |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:22页码:1706-1711 |
| 关键词 | BRAIN ISCHEMIA AKT NEUROPROTECTION EXPRESSION SURVIVAL DEATH ATP-sensitive potassium channel PC12 cells hypoxia apoptosis pinacidil phosphatidylinositol-3 kinase/v-akt murine thymoma viral oncogene homolog Bcl-2 |
| ISSN号 | 1673-5374 |
| 其他题名 | Adenosine triphosphate-sensitive potassium channel opener protects PC12 cells against hypoxia-induced apoptosis through PI3K/Akt and Bcl-2 signaling pathways |
| 英文摘要 | Although previous studies have shown the neuroprotective effects of the adenosine triphosphate (ATP)-sensitive potassium (K(ATP)) channel opener against ischemic neuronal damage, little is known about the mechanisms involved. Phosphatidylinositol-3 kinase (PI3K)/v-akt murine thymoma viral oncogene homolog (Akt) and Bcl-2 are thought to be important factors that mediate neuroprotection. The present study investigated the effects of K(ATP) openers on hypoxia-induced PC12 cell apoptosis, as well as mRNA and protein expression of Akt and Bcl-2. Results demonstrated that pretreatment of PC12 cells with pinacidil, a K(ATP) opener, resulted in decreased PC12 cell apoptosis following hypoxia, as detected by Annexin-V fluorescein isothiocyanate/propidium iodide double staining flow cytometry. In addition, mRNA and protein expression of phosphorylated Akt (p-Akt) and Bcl-2 increased, as detected by immunofluorescence, Western blot analysis, and reverse-transcription polymerase chain reaction. The protective effect of this preconditioning was attenuated by glipizide, a selective K(ATP) blocker. These results demonstrate for the first time that the protective mechanisms of K(ATP) openers on PC12 cell apoptosis following hypoxia could result from activation of the PI3K/Akt signaling pathway, which further activates expression of the downstream Bcl-2 gene. |
| 资助项目 | [Natural Science Foundation of Liaoning Province] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4095098 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/149366]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Hong,Jia Chunhong,Zhao Danyang,et al. Adenosine triphosphate-sensitive potassium channel opener protects PC12 cells against hypoxia-induced apoptosis through PI3K/Akt and Bcl-2 signaling pathways[J]. NEURAL REGENERATION RESEARCH,2010,5(22):1706-1711. |
| APA | Zhang Hong,Jia Chunhong,Zhao Danyang,Lu Yang,Wang Runling,&Li Jia.(2010).Adenosine triphosphate-sensitive potassium channel opener protects PC12 cells against hypoxia-induced apoptosis through PI3K/Akt and Bcl-2 signaling pathways.NEURAL REGENERATION RESEARCH,5(22),1706-1711. |
| MLA | Zhang Hong,et al."Adenosine triphosphate-sensitive potassium channel opener protects PC12 cells against hypoxia-induced apoptosis through PI3K/Akt and Bcl-2 signaling pathways".NEURAL REGENERATION RESEARCH 5.22(2010):1706-1711. |
入库方式： OAI收割
来源：金属研究所
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