Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia
文献类型：期刊论文
| 作者 | Yang Qian1; Gao Dianwen1; Nie Qingzhu1; Cai Zhengang2; Du Jian2; Shan Lujuan3; Liu Yuejian3 |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 6期号:25页码:1947-1953 |
| 关键词 | OXIDATIVE STRESS IN-VITRO EXPRESSION THERAPY BRAIN MICE TRANSCRIPTION ADAPTATION IMPROVES PROGRESS ultrasound-targeted microbubble destruction neuroglobin gene therapy recombinant plasmid SH-SY5Y cells neural regeneration |
| ISSN号 | 1673-5374 |
| 其他题名 | Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia |
| 英文摘要 | In this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm(2), a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4363499 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/149676]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.Dalian Med University, Hosp 1, Dept Gen Surg, Dalian 116021, Liaoning Provin, Peoples R China 3.Dalian Med University, Hosp 1, Expt Res Ctr, Dalian 116021, Liaoning Provin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Qian,Gao Dianwen,Nie Qingzhu,et al. Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia[J]. NEURAL REGENERATION RESEARCH,2011,6(25):1947-1953. |
| APA | Yang Qian.,Gao Dianwen.,Nie Qingzhu.,Cai Zhengang.,Du Jian.,...&Liu Yuejian.(2011).Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia.NEURAL REGENERATION RESEARCH,6(25),1947-1953. |
| MLA | Yang Qian,et al."Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia".NEURAL REGENERATION RESEARCH 6.25(2011):1947-1953. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。