Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism
文献类型：期刊论文
| 作者 | Chen Tiejun; Ha Minwen; Gong Yuehua; Xu Qian; Yuan Yuan |
| 刊名 | CHINESE JOURNAL OF CANCER RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:2页码:126-132 |
| 关键词 | APOPTOSIS EXPRESSION CARCINOMA CANCER allitridi stomach neoplasms cell cycle P34 cdc2/cyclin B(1) microtubule |
| ISSN号 | 1000-9604 |
| 其他题名 | Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism |
| 英文摘要 | Objective: To learn the effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanisms. Methods: We treated SGC-7901 cells with allitridi, and observed the proliferation inhibitory rate with MTT colometric assay, changes of cell cycle using flow cytometry and Switzerland-Giemsa's staining, and morphologic changes of the microtubule structure and location changes of cyclin B(1) expression using immunofluorescence and confocal laser scanning microscope. Furthermore, the expression of cyclin B(1) was analyzed quantitatively using Leica confocal software. Results: SGC-7901 cells were inhibited after exposure to allitridi and the IC(50) was 7.2 mu g/ml for 24 h, 20 mu g/ml for 72 h. When the cells were treated with allitridi at concentrations of 3, 6, and 9 mu g/ml for 24 h respectively, there was a declining tendency in the percentage of G(0)/G(1) cell but an increasing tendency in G(2)/M cell in the allitridi treated group compared with that of control (P < 0.01). When cells were treated allitridi at concentration of 6 mu g/ml for 24 h, its mitotic index was much higher (P < 0.01) than that of control, suggesting that allitridi caused arrest of gastric cancer cells in M phase. The cells were treated with allitridi became more shrunken and nepheloid, in which the microtubule networks disappeared, while the control cell exhibited an intact microtubule network. Contrasting with normal existence mainly in the cytoplasm, the cyclin B(1) was expressed more significantly and concentrated in the nucleus after exposure to allitridi. Fluorescence intensity of cyclin B1 protein in cells treated with allitridi was much more higher than that of control (P < 0.001). Conclusion: Allitridi can induce arrest of SGC-7901 cells in M phase, probably through enhancing microtubule depolymerization by elevating the expression of cyclin B(1). |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3299654 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/149795]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Chen Tiejun,Ha Minwen,Gong Yuehua,et al. Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(2):126-132. |
| APA | Chen Tiejun,Ha Minwen,Gong Yuehua,Xu Qian,&Yuan Yuan.(2008).Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism.CHINESE JOURNAL OF CANCER RESEARCH,20(2),126-132. |
| MLA | Chen Tiejun,et al."Effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901 and its possible mechanism".CHINESE JOURNAL OF CANCER RESEARCH 20.2(2008):126-132. |
入库方式： OAI收割
来源：金属研究所
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