Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA
文献类型：期刊论文
| 作者 | Liu MY1; Han W1; Ding YL1; Zhou TH1; Tian RY1; Yang SL1; Liu H1; Gong Y1 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:6页码:415-420 |
| 关键词 | B-LYMPHOCYTE STIMULATOR NECROSIS-FACTOR FAMILY AUTOIMMUNE-DISEASE SJOGRENS-SYNDROME CELL MATURATION TNF FAMILY BAFF MEMBER TACI MICE phage display neutralizing scFv antibody BLyS |
| ISSN号 | 1672-9145 |
| 其他题名 | Generation and Characterization of C305, a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA |
| 英文摘要 | B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2147594 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/149983]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院 2.暨南大学 3.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Liu MY,Han W,Ding YL,et al. Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(6):415-420. |
| APA | Liu MY.,Han W.,Ding YL.,Zhou TH.,Tian RY.,...&Gong Y.(2005).Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(6),415-420. |
| MLA | Liu MY,et al."Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.6(2005):415-420. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。