Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis
文献类型：期刊论文
| 作者 | Zhang Min1; Zhao Cong1; Du LianXiang1; Lu FuPing1; Gao Chen3 |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2008 |
| 卷号 | 51期号:1页码:52-59 |
| 关键词 | GENE CLONING LICHENIFORMIS SECRETION PLASMID ENZYME Bacillus subtilis thermophilic neutral protease expression purification characterization |
| ISSN号 | 1006-9305 |
| 其他题名 | Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis |
| 英文摘要 | The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65 degrees C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 degrees C. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3585990 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/150270]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnology, Tianjin 300457, Peoples R China 2.中国科学院金属研究所 3.南开大学 |
| 推荐引用方式 GB/T 7714 | Zhang Min,Zhao Cong,Du LianXiang,et al. Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(1):52-59. |
| APA | Zhang Min,Zhao Cong,Du LianXiang,Lu FuPing,&Gao Chen.(2008).Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(1),52-59. |
| MLA | Zhang Min,et al."Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.1(2008):52-59. |
入库方式： OAI收割
来源：金属研究所
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