The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF
文献类型：期刊论文
| 作者 | Han YL2; Xu HM2; Yan CH2; Hu Y2; Kang J2; Liu HW2 |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2006 |
| 卷号 | 33期号:5页码:438-445 |
| 关键词 | CELLS RECEPTOR repressor protein E1A phenotype cell vascular smooth muscle human |
| ISSN号 | 1000-3282 |
| 其他题名 | The Cellular Repressor of E1A-stimulated Genes Promotes Human VSMCs Differentiation In vitro Mediated by RhoA and SRF |
| 英文摘要 | To investigate the mechanism of the cellular repressor of E1A-stimulated genes (CREG) on the differentiation of vascular smooth muscle cells (VSMCs), the human internal thoracic artery cells (named HITASY cells) were stably infected with sense-CREG pLNCX(2)(+)/CREG and antisense-CREG pLXSN(-)/CREG retroviral vectors and positive clone was abtained by G418 selection. The cells infected with pLNCX2(+)/CREG take on the differentiated phenotype and overexpression of CREG can enhance SM alpha-actin expression accompanied with increase of the total RhoA and nuclear serum response factor (SRF) expression detected by immunofluorescence and Western blot. And treated with a selective Rho kinase inhibitor Y-27632, the expression of SM at-actin and nuclear SRF protein induced by CREG was effectively reduced. Meanwhile, the opposite results were observed in the CREG-depression HITASY cells. And immunoprecipitation assays indicate that as a secreted protein, CREG can be detected in the media of pLNCX2(+)/CREG cells and can interact with insulin-like growth factor II/mannose 6-phosphate receptor (M6P/IGF2R). Furthermore, that using okadaic acid (an inhibitor of the protein phosphatases 2A) to decrease the number of M6P/IGF2R at the cell surface can significantly inhibit the expression of total RhoA, nuclear SRF and SM a-actin induced by CREG-overexpression. Taken together, as a secreted protein, CREG can enhance the expression of SM alpha-actin by interaction with M6P/IGF2R and perform a pivotal role in the process of VSMCs differentiation and phenotype modulation. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2543256 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/151450]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院大连化学物理研究所 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Han YL,Xu HM,Yan CH,et al. The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(5):438-445. |
| APA | Han YL,Xu HM,Yan CH,Hu Y,Kang J,&Liu HW.(2006).The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(5),438-445. |
| MLA | Han YL,et al."The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.5(2006):438-445. |
入库方式： OAI收割
来源：金属研究所
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