Reversible histone acetylation involved in transcriptional regulation of WT1 gene
文献类型：期刊论文
| 作者 | Shao Yangguang2; Lu Jun2; Cheng Cao2; Cui Liguo2; Zhang Guoping2; Huang Baiqu2 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:12页码:931-938 |
| 关键词 | WILMS-TUMOR ACETYLTRANSFERASE P300 DEACETYLASE ENHANCER PROTEIN GATA-1 SP1 histone acetylation WT1 HDAC p300/CBP transcriptional regulation |
| ISSN号 | 1672-9145 |
| 其他题名 | Reversible Histone Acetylation Involved in Transcriptional Regulation of WT1 Gene |
| 英文摘要 | To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3088023 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/151457]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.上海市地震局 |
| 推荐引用方式 GB/T 7714 | Shao Yangguang,Lu Jun,Cheng Cao,et al. Reversible histone acetylation involved in transcriptional regulation of WT1 gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(12):931-938. |
| APA | Shao Yangguang,Lu Jun,Cheng Cao,Cui Liguo,Zhang Guoping,&Huang Baiqu.(2007).Reversible histone acetylation involved in transcriptional regulation of WT1 gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(12),931-938. |
| MLA | Shao Yangguang,et al."Reversible histone acetylation involved in transcriptional regulation of WT1 gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.12(2007):931-938. |
入库方式： OAI收割
来源：金属研究所
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