Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq
文献类型：期刊论文
| 作者 | Wang Shouming; Li Weijia; Liu Yuexue; Li He; Ma Yue; Zhang Zhihong |
| 刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| 出版日期 | 2017 |
| 卷号 | 16期号:4页码:828-844 |
| 关键词 | DE-NOVO CHARACTERIZATION GIBBERELLIN BIOSYNTHESIS GREEN-REVOLUTION DEFICIENT MUTANT CELL-DIVISION TOMATO MUTANT GENE ARABIDOPSIS PROTEIN GENOME strawberry fruit shape transcriptome RNA-Seq differentially expressed gene |
| ISSN号 | 2095-3119 |
| 其他题名 | Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq |
| 英文摘要 | Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (s.f). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sfl, and 28 differentially expressed GA pathway genes were identified between YW2 and sf2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5962859 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/152645]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Wang Shouming,Li Weijia,Liu Yuexue,et al. Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2017,16(4):828-844. |
| APA | Wang Shouming,Li Weijia,Liu Yuexue,Li He,Ma Yue,&Zhang Zhihong.(2017).Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq.JOURNAL OF INTEGRATIVE AGRICULTURE,16(4),828-844. |
| MLA | Wang Shouming,et al."Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq".JOURNAL OF INTEGRATIVE AGRICULTURE 16.4(2017):828-844. |
入库方式： OAI收割
来源：金属研究所
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