Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
文献类型：期刊论文
| 作者 | Wang Weiping1; Chai Ali2; Shi Yanxia2; Xie Xuewen2; Li Baoju2 |
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2015 |
| 卷号 | 35期号:5页码:1243-1247 |
| 关键词 | REAL-TIME PCR PLASMODIOPHORA-BRASSICAE CRUCIFEROUS CROPS RAPID DETECTION DISEASE Fourier transform infrared spectroscopy FTIR Chinese cabbage clubroot Plasmodiophora brassicae Model |
| ISSN号 | 1000-0593 |
| 其他题名 | Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy |
| 英文摘要 | Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm(-1) were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34. 17+12. 24x(1)-9. 81x(2)-6. 05x(3), r=0. 98 (p<0. 05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1. 60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5410956 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/152742]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.Chinese Acad Agr Sci, Institute Vegetables & Flowers, Beijing 100081, Peoples R China Institute Vegetables & Flowers |
| 推荐引用方式 GB/T 7714 | Wang Weiping,Chai Ali,Shi Yanxia,et al. Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2015,35(5):1243-1247. |
| APA | Wang Weiping,Chai Ali,Shi Yanxia,Xie Xuewen,&Li Baoju.(2015).Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,35(5),1243-1247. |
| MLA | Wang Weiping,et al."Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 35.5(2015):1243-1247. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。