Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress
文献类型：期刊论文
| 作者 | Wu Yingying1; Xue Bing2; Li Xiaojin1; Liu Hongchen1 |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:33页码:2583-2591 |
| 关键词 | SALVIANOLIC ACID B DIABETIC-NEUROPATHY ENDOTHELIAL-CELLS POLYOL PATHWAY GROWTH-FACTOR IN-VITRO RAT PROLIFERATION COMPLICATIONS ACTIVATION puerarin diabetic peripheral neuropathy hyperglycemia Schwann cell apoptosis caspase mitochondrial transmembrane potential oxidative stress 8-hydroxy-2-deoxyguanosine reactive oxygen radical |
| ISSN号 | 1673-5374 |
| 其他题名 | Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress ★ |
| 英文摘要 | Oxidative stress may be the unifying factor for the injury caused by hyperglycemia in diabetic peripheral neuropathy. Puerarin is the major isoflavonoid derived from Radix puerariae and has been shown to be effective in increasing superoxide dismutase activity. This study sought to investigate the neuroprotective effect of puerarin on high glucose-induced oxidative stress and Schwann cell apoptosis in vitro. Intracellular reactive oxygen radicals and mitochondrial transmembrane potential were detected by flow cytometry analysis. Apoptosis was confirmed by TUNEL and oxidative stress was monitored using an enzyme-linked immunosorbent assay for the DNA marker 8-hydroxy-2-deoxyguanosine. The expression levels of bax and bcl-2 were analyzed by quantitative real-time reverse transcriptase-PCR, while protein expression of cleaved caspase-3 and -9 were analyzed by means cif western blotting. Results suggested that puerarin treatment inhibited high glucose-induced oxidative stress, mitochondrial depolarization and apoptosis in a dose-dependent manner. Furthermore, puerarin treatment downregulated Bax expression, upregulated bcl-2 expression and attenuated the activation of caspase-3 and -9. Overall, our results indicated that puerarin antagonized high glucose-induced oxidative stress and apoptosis in Schwann cells. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4694391 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/153044]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国人民解放军总医院 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Wu Yingying,Xue Bing,Li Xiaojin,et al. Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress[J]. NEURAL REGENERATION RESEARCH,2012,7(33):2583-2591. |
| APA | Wu Yingying,Xue Bing,Li Xiaojin,&Liu Hongchen.(2012).Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress.NEURAL REGENERATION RESEARCH,7(33),2583-2591. |
| MLA | Wu Yingying,et al."Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress".NEURAL REGENERATION RESEARCH 7.33(2012):2583-2591. |
入库方式： OAI收割
来源：金属研究所
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