Synthesis of novel hydrophobic media and purification of recombinant HBsAg
文献类型：期刊论文
| 作者 | Wang Y3; Wang Q3; Xiao NQ3; Liu DW3; Ye SD3; Luo X3; Guan GF3; Wei ZL3; Chen WG3; Bai F3 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 1999 |
| 卷号 | 15期号:1页码:39-46 |
| 关键词 | Chinese hamster ovary (CHO) recombinant hepatitis B surface antigen (r-HBsAg) hydrophobic interaction chromatography (HIC) reverse phase haemagglutination assay (RPHA) |
| ISSN号 | 1005-9040 |
| 其他题名 | Synthesis of novel hydrophobic media and purification of recombinant HBsAg |
| 英文摘要 | A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r-HBsAg). r-HBsAg was further purified to the final products by following a downstream procedure(1). The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r-HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r-HBsAg products have reached the same level as other similar kinds of products. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:496710 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/153340]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.北京大学 3.中山大学 |
| 推荐引用方式 GB/T 7714 | Wang Y,Wang Q,Xiao NQ,et al. Synthesis of novel hydrophobic media and purification of recombinant HBsAg[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,1999,15(1):39-46. |
| APA | Wang Y.,Wang Q.,Xiao NQ.,Liu DW.,Ye SD.,...&Bai F.(1999).Synthesis of novel hydrophobic media and purification of recombinant HBsAg.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,15(1),39-46. |
| MLA | Wang Y,et al."Synthesis of novel hydrophobic media and purification of recombinant HBsAg".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 15.1(1999):39-46. |
入库方式： OAI收割
来源：金属研究所
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