Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells
文献类型：期刊论文
| 作者 | Yu Xiuling; Gao Dianwen |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:23页码:2198-2203 |
| 关键词 | INDUCIBLE FACTOR-1 NEUROGLOBIN EXPRESSION BRAIN TISSUES ANOXIA DEATH neural regeneration cytoglobin hypoxia green fluorescent protein transfection SH-SY5Y cells cobalt chloride recombinant plasmid neuroprotection neuroregeneration |
| ISSN号 | 1673-5374 |
| 其他题名 | Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells |
| 英文摘要 | A plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1 alpha in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible factor-1 alpha expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increasing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-1 alpha are strongly up-regulated by hypoxia, and that there is a strong relationship between hypoxia-inducible factor-1 alpha and cytoglobin during hypoxic injury. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4916341 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/154097]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Yu Xiuling,Gao Dianwen. Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells[J]. NEURAL REGENERATION RESEARCH,2013,8(23):2198-2203. |
| APA | Yu Xiuling,&Gao Dianwen.(2013).Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells.NEURAL REGENERATION RESEARCH,8(23),2198-2203. |
| MLA | Yu Xiuling,et al."Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells".NEURAL REGENERATION RESEARCH 8.23(2013):2198-2203. |
入库方式： OAI收割
来源：金属研究所
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