Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study
文献类型：期刊论文
| 作者 | Zhang Chao3; Wang Lu2; Guan Xin3; Fawcett J Paul1; Zhao Limei2; Sun Yantong3; Gu Jingkai3 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2010 |
| 卷号 | 26期号:6页码:910-914 |
| 关键词 | FAMOTIDINE HPLC BIOEQUIVALENCE METABOLITES NIZATIDINE VOLUNTEERS ASSAY Ranitidine Pharmacokinetics Human plasma Protein precipitation LC-MS/MS |
| ISSN号 | 1005-9040 |
| 其他题名 | Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study |
| 英文摘要 | A rapid and sensitive assay based on high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of ranitidine(RAN) in human plasma with codeine as internal standard(IS). After protein precipitation with acetonitrile, the analyte and IS were separated on a Zorbax SB-Aq C-18 column(150 mmx4.6 mm i. d., 5 mu m) eluted with a mobile phase consisting of methanol/acetonitrile/10 mmol/L ammonium acetate containing 1% formic acid(pH=2.4)(volume ratio 12.5:12.5:75) at a flow rate of 1.0 mL/min. Detection was performed by electrospray ionization in the positive ion mode followed by the multiple reaction monitoring(MRM) of the transitions of RAN at m/z 315.1 -> 176.3 and of IS at m/z 300.1 -> 165.1. The method was linear over a concentration range of 1-1000 ng/mL(r=0.9991) with a lower limit of quantitation(LLOQ) of 1 ng/mL and a limit of detection(LOD) of 0.3 ng/mL. Accuracy as relative error was from -0.01% to -1.7% and intra-day and inter-day precisions as relative standard deviation were <= 8.9% and <= 5.5%, respectively. The method was successfully applied to a pharmacokinetic study of ranitidine, getting a single oral dose(160 mg) to healthy volunteers. |
| 资助项目 | [National High-Tech Research and Development Program of China] ; [National Natural Science Foundation of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4093582 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/154155]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.University Otago, Sch Pharm, Dunedin, New Zealand 2.中国科学院金属研究所 3.吉林大学 |
| 推荐引用方式 GB/T 7714 | Zhang Chao,Wang Lu,Guan Xin,et al. Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2010,26(6):910-914. |
| APA | Zhang Chao.,Wang Lu.,Guan Xin.,Fawcett J Paul.,Zhao Limei.,...&Gu Jingkai.(2010).Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,26(6),910-914. |
| MLA | Zhang Chao,et al."Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 26.6(2010):910-914. |
入库方式： OAI收割
来源：金属研究所
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