In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells
文献类型：期刊论文
| 作者 | Zhao HongBo5; Wang YaShu3; Jiang XueMei4; Shi XiaoHao5; Zhong HongZhe2; Wang YaJie1; Chen Jun4; Deng LinHong5 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2013 |
| 卷号 | 58期号:21页码:2576-2583 |
| 关键词 | MUSCLE CELLS ACTIN CYTOTOXICITY APOPTOSIS HepG2 cells F-actin cytoskeleton nanomechanics tubeimoside I anticancer drug screening |
| ISSN号 | 1001-6538 |
| 其他题名 | In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells |
| 英文摘要 | Cytoskeleton nanomechanics characterizes cancer cell's physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the F-actin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G') of HepG2 cells was affected by TBMS I. G' exhibited a typical power law with respect to the loading frequency (f), i.e. g similar to f (alpha) . The magnitude of G' and the value of exponent (alpha) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of F-actin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates. |
| 资助项目 | [National Natural Science Foundation of China] ; [Training Program for Hundreds of Distinguished Leading Scientists of Chongqing] ; [Chongqing Natural Science Foundation] ; [Fundamental Research Funds for the Central Universities] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4877268 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/154161]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Chinese Peoples Liberat Army, Hosp 324, Chongqing 400020, Peoples R China Hosp 324 2.中国科学院金属研究所 3.Chinese Peoples Armed Police Forces, Xinjiang Prov Corps Hosp, Urumqi 830002, Peoples R China Xinjiang Prov Corps Hosp 4.重庆大学 5.Changzhou Univ, Institute Biomed Engn & Hlth Sci, Changzhou 213164, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao HongBo,Wang YaShu,Jiang XueMei,et al. In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells[J]. CHINESE SCIENCE BULLETIN,2013,58(21):2576-2583. |
| APA | Zhao HongBo.,Wang YaShu.,Jiang XueMei.,Shi XiaoHao.,Zhong HongZhe.,...&Deng LinHong.(2013).In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells.CHINESE SCIENCE BULLETIN,58(21),2576-2583. |
| MLA | Zhao HongBo,et al."In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells".CHINESE SCIENCE BULLETIN 58.21(2013):2576-2583. |
入库方式： OAI收割
来源：金属研究所
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