Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification
文献类型：期刊论文
| 作者 | Zhang Bin2; Jiang Jiquan2; Yuan Ying2; Guan Yifu2 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2019 |
| 卷号 | 35期号:6页码:1119-1123 |
| ISSN号 | 1005-9040 |
| 关键词 | DNA DETECTION QUADRUPLEX IDENTIFICATION STABILITY Rolling circle amplification SYBR Green II Nucleotide-biased Fluorescence emission |
| 英文摘要 | The fluorescence dye SYBR Green II(SG II) has been frequently used in rolling circle amplification(RCA) based analyses of nucleic acids. However, a good amount of inconsistencies have been reported in regards the quality and reproducibility of RCA reactions. To properly examine this experimental issue, here we utilized a series of synthetic oligonucleotides and circular templates to investigate the impact of SG II in RCA reactions. The results indicate that SG II enables a strong fluorescence signal only when complexing with guanosine(G) residue. In RCA reactions, long single-stranded RCA products, enriched with G residues, result in higher fluorescence emission when compared with the addition of other nucleotide residues. These results suggest that the nucleotide composition of the reaction can affect the amplification results and, eventually, can lead to inconsistent fluorescence of the RCA products. This work indicates that particular attention should be given when circular templates are designed for the quantitative analysis of nucleic acids, to further allow the signal reproducibility of RCA-based experiments. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6626732 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/155542]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.中国科学院地质与地球物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Bin,Jiang Jiquan,Yuan Ying,et al. Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2019,35(6):1119-1123. |
| APA | Zhang Bin,Jiang Jiquan,Yuan Ying,&Guan Yifu.(2019).Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,35(6),1119-1123. |
| MLA | Zhang Bin,et al."Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 35.6(2019):1119-1123. |
入库方式： OAI收割
来源：金属研究所
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