Genome-wide characterization of long terminal repeat-retrotransposons in apple reveals the differences in heterogeneity and copy number between Ty1-copia and Ty3-gypsy retrotransposons
文献类型：期刊论文
| 作者 | Sun HaiYue2; Dai HongYan2; Zhao GuiLing2; Ma Yue2; Ou ChunQing2; Li He2; Li LinGuang2; Zhang ZhiHong2 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 50期号:9页码:1130-1139 |
| 关键词 | REVERSE-TRANSCRIPTASE DOMAIN PLANT TRANSPOSABLE ELEMENTS PHYLOGENETIC ANALYSIS LTR RETROTRANSPOSONS SEQUENCE ALIGNMENT METHYL JASMONATE CITRUS-SINENSIS TISSUE-CULTURE GYPSY-TYPE EVOLUTION heterogeneity Malus domestica retrotransposon reverse transcriptase transcriptional activity |
| ISSN号 | 1672-9072 |
| 其他题名 | Genome-wide Characterization of Long Terminal Repeat -retrotransposons in Apple Reveals the Differences in Heterogeneity and Copy Number between Ty1-copia and Ty3-gypsy Retrotransposons |
| 英文摘要 | The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were isolated from the Malus domestica genome using degenerate oligonucleotide primers. Sequence analysis showed that 45% of Ty1-copia and 63% of Ty3-gypsy RT sequences contained premature stop codons and/or indels disrupting the reading frame. High heterogeneity among RT sequences of both Ty1-copia and Ty3-gypsy group retrotransposons was observed, but Ty3-gypsy group retrotransposons in the apple genome are less heterogeneous than Ty1-copia elements. Retrotransposon copy number was estimated by dot blot hybridizations for Ty1-copia (similar to 5 000) and Ty3-gypsy (similar to 26 000). All elements of the two types of LTR retrotransposons comprise approximately 38% of the M. domestica genome, with the Ty3-gypsy group contribution being higher (33.5%) than the Ty1-copia one (4.6%). Transcription was not detected by reverse transcription-polymerase chain reaction for either Ty1-copia or Ty3-gypsy retrotransposons in the leaves of plants in vitro or in leaf explants cultured on medium supplemented with high concentration benzylaminopurine. This research reveals the differences in heterogeneity and copy number between Ty1-copia and Ty3-gypsy retrotransposons in the apple genome. Ty1-copia retrotransposon has higher heterogeneity than Ty3-gypsy retrotransposon, but the latter has a higher copy number, which implies that Ty3-gypsy retrotransposons may play a more important role in the apple genome evolution. |
| 资助项目 | [National Natural Science Foundation of China] ; [Specialized Research Fund for the Doctoral Program of Higher Education] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3324769 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/156219]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Shandong Institute Pomol, Tai An 271000, Shandong, Peoples R China 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Sun HaiYue,Dai HongYan,Zhao GuiLing,et al. Genome-wide characterization of long terminal repeat-retrotransposons in apple reveals the differences in heterogeneity and copy number between Ty1-copia and Ty3-gypsy retrotransposons[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2008,50(9):1130-1139. |
| APA | Sun HaiYue.,Dai HongYan.,Zhao GuiLing.,Ma Yue.,Ou ChunQing.,...&Zhang ZhiHong.(2008).Genome-wide characterization of long terminal repeat-retrotransposons in apple reveals the differences in heterogeneity and copy number between Ty1-copia and Ty3-gypsy retrotransposons.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,50(9),1130-1139. |
| MLA | Sun HaiYue,et al."Genome-wide characterization of long terminal repeat-retrotransposons in apple reveals the differences in heterogeneity and copy number between Ty1-copia and Ty3-gypsy retrotransposons".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 50.9(2008):1130-1139. |
入库方式： OAI收割
来源：金属研究所
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