Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells
文献类型：期刊论文
| 作者 | Li Xiuqin; Ren Bo; Du Zhenhua |
| 刊名 | CHINESE JOURNAL OF CANCER RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:3页码:186-189 |
| 关键词 | SURFACE EPITHELIUM GROWTH-FACTOR BCL-2 FAMILY EXPRESSION APOPTOSIS INHIBIN Ovarian cancer OVCAR-3 cells line activin A bcl-2 protein MTT |
| ISSN号 | 1000-9604 |
| 其他题名 | Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells |
| 英文摘要 | Objective: To investigate the proliferation effect and its pathway of activin A on Ovarian epithelial cancer cells line OVCAR-3. Methods: OVCAR-3 cells were cultured in vitro and the membrane receptor ActR II was detected by immunohistochemical method. The OVCAR-3 cells were cultured with 10 ng/ml activin A for 7 d to observe the effects. Activin A at 5, 10, 15 and 20 ng/ml was used separately to treat the OVCAR-3 cancer cells for 24, 48 and 72 h in order to draw the growth proliferation rate curve measured by MTT method. The expression of protein bcl-2 was detected by western-blot. When OVCAR-3 cells were treated with 10 ng/ml activin A and 5 mu g/ml DDP for 24, 48 and 72 h, cell apoptosis could be detected by electron microscopy and flow cytometry (FCM). Results: Positive expression of ActR II was detected. We also found that the proliferation of OVCAR-3 reached to the climax on the 5th day of culture. The experiments showed that the cells treated with activin A increased quickly and grew faster than those in control group. Moreover, OVCAR-3 cells treated with activin A for 48 h proliferated significantly greater than those treated for 24 h or 72 h (P < 0.01). bcl-2 protein expression increased expression in activin A treated group than in control group (P < 0.05). Conclusion: Activin A could increase the proliferation of OVCAR-3 cells which may be through bcl-2 anti-apoptosis pathway. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3360139 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/156349]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Li Xiuqin,Ren Bo,Du Zhenhua. Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):186-189. |
| APA | Li Xiuqin,Ren Bo,&Du Zhenhua.(2008).Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells.CHINESE JOURNAL OF CANCER RESEARCH,20(3),186-189. |
| MLA | Li Xiuqin,et al."Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):186-189. |
入库方式： OAI收割
来源：金属研究所
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