Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)
文献类型：期刊论文
| 作者 | Shu Yang2; Meng Ying2; Chen MingLi2; Wang JianHua2 |
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2015 |
| 卷号 | 26期号:12页码:1460-1464 |
| 关键词 | SELECTIVE ISOLATION COMPOSITES ENRICHMENT ADSORPTION PROTEINS Metal-organic frameworks Hemoglobin Solid-phase extraction Protein solation |
| ISSN号 | 1001-8417 |
| 其他题名 | Isolation of hemoglobin with metal–organic frameworks Y(BTC)(H2O)6 |
| 英文摘要 | The hierarchical metal-organic frameworks (MOFs), such as Y(BTC)(H2O)(6), are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H2O)(6) particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H2O)(6) fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g(-1). An adsorption efficiency of 87.7% for 100 mu g mL(-1) hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H2O)(6). 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved. |
| 资助项目 | [National Natural Science Foundation of China] ; [Fundamental Research Funds for the Central Universities] |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5605583 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/156555]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.东北大学 |
| 推荐引用方式 GB/T 7714 | Shu Yang,Meng Ying,Chen MingLi,et al. Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)[J]. CHINESE CHEMICAL LETTERS,2015,26(12):1460-1464. |
| APA | Shu Yang,Meng Ying,Chen MingLi,&Wang JianHua.(2015).Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6).CHINESE CHEMICAL LETTERS,26(12),1460-1464. |
| MLA | Shu Yang,et al."Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)".CHINESE CHEMICAL LETTERS 26.12(2015):1460-1464. |
入库方式： OAI收割
来源：金属研究所
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