RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid
文献类型：期刊论文
| 作者 | Li K1; Bi KS1 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2006 |
| 卷号 | 22期号:1页码:56-60 |
| 关键词 | Di-Gu-Pi decoction cinnamic acid pharmacokinetics RP-HPLC |
| ISSN号 | 1005-9040 |
| 其他题名 | RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid |
| 英文摘要 | A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase (HPLC) system with a Diamonsil C, column and methanol-acetonitrile-water (8:32:60, volume ratio) ( adjusted to pH = 3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 mu g/mL (R-2 = 0.9988, n = 9). The precision was 3.42%-10.10% ; the between-day precision was 2.84%-8.91%; the accuracy was 1.51%-1.26% the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2659490 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/157132]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Beijing University 2.中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Li K,Bi KS. RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(1):56-60. |
| APA | Li K,&Bi KS.(2006).RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,22(1),56-60. |
| MLA | Li K,et al."RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 22.1(2006):56-60. |
入库方式： OAI收割
来源：金属研究所
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