Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration
文献类型：期刊论文
| 作者 | Wang M2; Li QR2 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:6页码:703-706 |
| 关键词 | CATHARANTHUS-ROSEUS TRYPTOPHAN DECARBOXYLASE NICOTIANA-TABACUM CELL-CULTURES SEQUENCE GENES strictosidine synthase vacuum infiltration subcellular compartments Nicotiana tabacum |
| ISSN号 | 0582-9879 |
| 其他题名 | Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration |
| 英文摘要 | Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1141013 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/157678]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.中国科学院金属研究所 2.中国科学院 |
| 推荐引用方式 GB/T 7714 | Wang M,Li QR. Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):703-706. |
| APA | Wang M,&Li QR.(2002).Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),703-706. |
| MLA | Wang M,et al."Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):703-706. |
入库方式： OAI收割
来源：金属研究所
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