MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells
文献类型：期刊论文
| 作者 | Cao Sunqiong; Ren Changshan |
| 刊名 | CHINESE JOURNAL OF CANCER RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:3页码:159-163 |
| 关键词 | OVEREXPRESSION TRANSFORMATION CONTRIBUTES CANCER TRF1 NBS1 gene RNA interfere MicroRNA Telomerase activation |
| ISSN号 | 1000-9604 |
| 其他题名 | MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells |
| 英文摘要 | Objective: To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela. Methods: According to the sequence of NBS1 mRNA, the NBS1 pre-microRNA was designed and synthesized, then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cells. The integrity of the insert fragment was verified through colony PCR and sequencing analysis. The NBS1 gene expression of NBS1 microRNA recombinants was detected by Real-Time PCR and western blot. Telomerase activity in Hela cells was assayed by TRAP-PCR-EB. Results: Sequences of insert fragment in microRNA expressing recombinants were correct. The NBS1 gene expression was decreased, and the telomerase activation of Hela cell reduced. Conclusion: NBS1 microRNA inhibits NBS1 gene expression, and depresses telomerase activation of Hela cells. This confirms that there is relevance between NBS1 gene and telomerase activity. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3360134 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/157956]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Cao Sunqiong,Ren Changshan. MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):159-163. |
| APA | Cao Sunqiong,&Ren Changshan.(2008).MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells.CHINESE JOURNAL OF CANCER RESEARCH,20(3),159-163. |
| MLA | Cao Sunqiong,et al."MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):159-163. |
入库方式： OAI收割
来源：金属研究所
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