Physaria fendleri and Ricinus communis lecithin:cholesterol acyltransferase-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
文献类型:期刊论文
作者 | Xu, Yang7; Caldo, Kristian Mark P.; Singer, Stacy D.; Mietkiewska, Elzbieta; Greer, Michael S.; Tian, Bo2; Dyer, John M.; Smith, Mark4; Zhou, Xue-Rong; Qiu, Xiao6 |
刊名 | PLANT JOURNAL |
出版日期 | 2021 |
卷号 | 105期号:1页码:182-196 |
ISSN号 | 0960-7412 |
关键词 | FATTY-ACID ACCUMULATION SEED OIL CONTENT CASTOR EXPRESSION GENE IDENTIFICATION METABOLISM MEMBRANE TRANSFORMATION GLYCEROLIPIDS |
DOI | 10.1111/tpj.15050 |
英文摘要 | Production of hydroxy fatty acids (HFAs) in transgenic crops represents a promising strategy to meet our demands for specialized plant oils with industrial applications. The expression of Ricinus communis (castor) OLEATE 12-HYDROXYLASE (RcFAH12) in Arabidopsis has resulted in only limited accumulation of HFAs in seeds, which probably results from inefficient transfer of HFAs from their site of synthesis (phosphatidylcholine; PC) to triacylglycerol (TAG), especially at the sn-1/3 positions of TAG. Phospholipase As (PLAs) may be directly involved in the liberation of HFAs from PC, but the functions of their over-expression in HFA accumulation and distribution at TAG in transgenic plants have not been well studied. In this work, the functions of lecithin:cholesterol acyltransferase-like PLAs (LCAT-PLAs) in HFA biosynthesis were characterized. The LCAT-PLAs were shown to exhibit homology to LCAT and mammalian lysosomal PLA(2), and to contain a conserved and functional Ser/His/Asp catalytic triad. In vitro assays revealed that LCAT-PLAs from the HFA-accumulating plant species Physaria fendleri (PfLCAT-PLA) and castor (RcLCAT-PLA) could cleave acyl chains at both the sn-1 and sn-2 positions of PC, and displayed substrate selectivity towards sn-2-ricinoleoyl-PC over sn-2-oleoyl-PC. Furthermore, co-expression of RcFAH12 with PfLCAT-PLA or RcLCAT-PLA, but not Arabidopsis AtLCAT-PLA, resulted in increased occupation of HFA at the sn-1/3 positions of TAG as well as small but insignificant increases in HFA levels in Arabidopsis seeds compared with RcFAH12 expression alone. Therefore, PfLCAT-PLA and RcLCAT-PLA may contribute to HFA turnover on PC, and represent potential candidates for engineering the production of unusual fatty acids in crops. |
学科主题 | Plant Sciences |
语种 | 英语 |
WOS记录号 | WOS:000592434800001 |
源URL | [http://ir.xtbg.org.cn/handle/353005/11954] |
专题 | 西双版纳热带植物园_药用植物栽培技术组 |
作者单位 | 1.Univ Alberta, Agr Forestry Ctr 410, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada 2.Singer, Stacy D.] Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB T1J 4B1, Canada 3.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Plant Resource & Sustainable Use, Kunming 650223, Yunnan, Peoples R China 4.Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA 5.Agr & Agri Food Canada, 107 Sci Pl, Saskatoon, SK S7N 0X2, Canada 6.CSIRO Agr & Food, POB 1700, Canberra, ACT 2601, Australia 7.Univ Saskatchewan, Dept Food & Bioprod Sci, Saskatoon, SK S7N 5A8, Canada 8.Michigan State Univ, MSU DOE Plant Res Lab, E Lansing, MI 48824 USA 9.Caldo, Kristian Mark P.] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada |
推荐引用方式 GB/T 7714 | Xu, Yang,Caldo, Kristian Mark P.,Singer, Stacy D.,et al. Physaria fendleri and Ricinus communis lecithin:cholesterol acyltransferase-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine[J]. PLANT JOURNAL,2021,105(1):182-196. |
APA | Xu, Yang.,Caldo, Kristian Mark P..,Singer, Stacy D..,Mietkiewska, Elzbieta.,Greer, Michael S..,...&Chen, Guanqun.(2021).Physaria fendleri and Ricinus communis lecithin:cholesterol acyltransferase-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine.PLANT JOURNAL,105(1),182-196. |
MLA | Xu, Yang,et al."Physaria fendleri and Ricinus communis lecithin:cholesterol acyltransferase-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine".PLANT JOURNAL 105.1(2021):182-196. |
入库方式: OAI收割
来源:西双版纳热带植物园
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