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Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC

文献类型：期刊论文

作者Li, Ya-Nan1; Li, Jia-Nan2,4,5,6; Ouyang, Qin1; Zhou, Yu-Bo2,4,5,6; Lei, Chun1; Cui, Ming-Jun3; Fu, Kai-Cong3; Li, Jia2,4,5,6; Huang, Jian-Ming1; Hou, Ai-Jun1
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2021-05-10
卷号198页码:7
关键词Trewia nudiflora QuEChERS method Maytansinoids HPLC
ISSN号0731-7085
DOI10.1016/j.jpba.2021.113993
通讯作者Zhou, Yu-Bo(ybzhou@simm.ac.cn) ; Li, Jia(jli@simm.ac.cn) ; Huang, Jian-Ming(jmhuang@shmu.edu.cn) ; Hou, Ai-Jun(ajhou@shmu.edu.cn)
英文摘要Three maytansinoids with strong cytotoxicities, dehydrotrewiasine, maytanbutine, and trewiasine, were isolated and identified from Trewia nudiflora, and maytanbutine was obtained from this plant for the first time. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction combined with high-performance liquid chromatography (HPLC) was established to determine the three maytansinoids in T. nudiflora. The effects of major factors on the extraction efficiency of the QuEChERS method were evaluated and the optimal conditions using acetonitrile-ethyl acetate (1:1, v/v) as the extraction solvent and PestiCarb as the clean-up sorbents were established. Compared with Soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE), the QuEChERS method was easy-to-operate and afforded a cleaner extract. A phenomenex HyperClone BDS C-18 column was used for HPLC analysis. Methanol-acetonitrile-water was chosen as mobile phase for gradient elution. Method validation showed that all analytes showed good linearity (r > 0.999) over the investigated ranges and satisfactory recoveries ranging from 95.0% to 105.0%. The developed QuEChERS-HPLC method was simple, efficient, and applicable to the determination of maytansinoids in T. nudiflora. (C) 2021 Elsevier B.V. All rights reserved.
资助项目Shanghai Commissionof Science and Technology[19495800800] ; National Natural Science Foundation of China[21672040] ; National Natural Science Foundation of China[82073717]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000636654000012
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/295249]  
专题新药研究国家重点实验室
通讯作者Zhou, Yu-Bo; Li, Jia; Huang, Jian-Ming; Hou, Ai-Jun
作者单位1.Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Peoples R China
3.Puer Inst Tradit Ethnomed, Puer 665000, Yunnan, Peoples R China
4.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
推荐引用方式
GB/T 7714		 Li, Ya-Nan,Li, Jia-Nan,Ouyang, Qin,et al. Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2021,198:7.
APA Li, Ya-Nan.,Li, Jia-Nan.,Ouyang, Qin.,Zhou, Yu-Bo.,Lei, Chun.,...&Hou, Ai-Jun.(2021).Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,198,7.
MLA Li, Ya-Nan,et al."Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 198(2021):7.

入库方式： OAI收割

来源：上海药物研究所

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