An enhanced strategy integrating offline superimposed two-dimensional separation with mass defect filter and diagnostic ion filter: Comprehensive characterization of steroid alkaloids in Fritillariae Pallidiflorae Bulbus as a case study
文献类型：期刊论文
| 作者 | An, Ya-ling1,2; Wei, Wen-long1; Li, Hao-jv1,2; Li, Zhen-wei1,2; Yao, Chang-liang1; Qu, Hua1; Yao, Shuai1; Huang, Yong1; Zhang, Jian-qing1; Bi, Qi-rui1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2021-04-26 |
| 卷号 | 1643页码:10 |
| 关键词 | Mass defect filter Diagnostic ion filter Comprehensive characterization Steroid alkaloids Fritillariae Pallidiflorae Bulbus Offline superimposed two-dimensional separation |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2021.462029 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | The inherent complexity of traditional Chinese medicines necessitates the application of multidimensional information to accomplish comprehensive profiling and confirmative identification of their chemical components. In this study, we display an enhanced strategy by integrating offline superimposed two-dimensional separation (S-2D-LC) with mass defect filter and diagnostic ion filter to comprehensively characterize the alkaloid composition of Fritillariae Pallidiflorae Bulbus (FPB). The superimposed HILIC & times; RP and UPCC & times; RP offline two-dimensional liquid chromatography system was constructed with superior orthogonality ( R 2 = 0.004 and R 2 = 0.001) for chromatographic separation. In total, 70 fractions were collected after the first-dimensional chromatographic separation (HILIC and UPCC) and then analyzed by the second-dimensional reversed phase (RP) liquid chromatography coupled with Q-TOF/MS/MS in FAST DDA acquisition mode. A four-step interpretation strategy combining mass defect filter with diagnostic ion filter was developed to rapidly characterize alkaloids in Fritillaria species. Ultimately, a sum of 529 Fritillaria alkaloids were characterized from two botanical origins of FPB. The integrated strategy is practical to efficiently expose and comprehensively characterize more trace and isomeric components in complex herbal medicines. (c) 2021 Published by Elsevier B.V. The inherent complexity of traditional Chinese medicines necessitates the application of multidimensional information to accomplish comprehensive profiling and confirmative identification of their chemical components. In this study, we display an enhanced strategy by integrating offline superimposed two-dimensional separation (S-2D-LC) with mass defect filter and diagnostic ion filter to comprehensively characterize the alkaloid composition of Fritillariae Pallidiflorae Bulbus (FPB). The superimposed HILIC ? RP and UPCC ? RP offline two-dimensional liquid chromatography system was constructed with superior orthogonality ( R 2 = 0.004 and R 2 = 0.001) for chromatographic separation. In total, 70 fractions were collected after the first-dimensional chromatographic separation (HILIC and UPCC) and then analyzed by the second-dimensional reversed phase (RP) liquid chromatography coupled with Q-TOF/MS/MS in FAST DDA acquisition mode. A four-step interpretation strategy combining mass defect filter with diagnostic ion filter was developed to rapidly characterize alkaloids in Fritillaria species. Ultimately, a sum of 529 Fritillaria alkaloids were characterized from two botanical origins of FPB. The integrated strategy is practical to efficiently expose and comprehensively characterize more trace and isomeric components in complex herbal medicines. |
| WOS关键词 | IDENTIFICATION ; SPECTROMETRY ; EXTRACTION |
| 资助项目 | National Key R&D Program of China[2019YFC1711000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH-K-LN-01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000632479100002 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/295372]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Haike Rd 501, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Ya-ling,Wei, Wen-long,Li, Hao-jv,et al. An enhanced strategy integrating offline superimposed two-dimensional separation with mass defect filter and diagnostic ion filter: Comprehensive characterization of steroid alkaloids in Fritillariae Pallidiflorae Bulbus as a case study[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1643:10. |
| APA | An, Ya-ling.,Wei, Wen-long.,Li, Hao-jv.,Li, Zhen-wei.,Yao, Chang-liang.,...&Guo, De-an.(2021).An enhanced strategy integrating offline superimposed two-dimensional separation with mass defect filter and diagnostic ion filter: Comprehensive characterization of steroid alkaloids in Fritillariae Pallidiflorae Bulbus as a case study.JOURNAL OF CHROMATOGRAPHY A,1643,10. |
| MLA | An, Ya-ling,et al."An enhanced strategy integrating offline superimposed two-dimensional separation with mass defect filter and diagnostic ion filter: Comprehensive characterization of steroid alkaloids in Fritillariae Pallidiflorae Bulbus as a case study".JOURNAL OF CHROMATOGRAPHY A 1643(2021):10. |
入库方式： OAI收割
来源：上海药物研究所
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