Structural insights into the human D1 and D2 dopamine receptor signaling complexes
文献类型：期刊论文
| 作者 | Zhuang, Youwen1,2; Xu, Peiyu1,2,3,4; Mao, Chunyou3,4,5,6; Wang, Lei7; Krumm, Brian8; Zhou, X. Edward9; Huang, Sijie1,2,10; Liu, Heng7; Cheng, Xi1,11; Huang, Xi-Ping8 |
| 刊名 | CELL
 |
| 出版日期 | 2021-02-18 |
| 卷号 | 184期号:4页码:931-+ |
| ISSN号 | 0092-8674 |
| DOI | 10.1016/j.cell.2021.01.027 |
| 通讯作者 | Roth, Bryan L.(bryan_roth@med.unc.edu) ; Zhang, Yan(zhang_yan@zju.edu.cn) ; Zhang, Cheng(chengzh@pitt.edu) ; Xu, H. Eric(eric.xu@simm.ac.cn) |
| 英文摘要 | The D1- and D2-dopamine receptors (D1R and D2R), which signal through G(s) and G(i), respectively, represent the principal stimulatory and inhibitory dopamine receptors in the central nervous system. D1R and D2R also represent the main therapeutic targets for Parkinson's disease, schizophrenia, and many other neuropsychiatric disorders, and insight into their signaling is essential for understanding both therapeutic and side effects of dopaminergic drugs. Here, we report four cryoelectron microscopy (cryo-EM) structures of D1R-G(s) and D2R-G(i) signaling complexes with selective and non-selective dopamine agonists, including two currently used anti-Parkinson's disease drugs, apomorphine and bromocriptine. These structures, together with mutagenesis studies, reveal the conserved binding mode of dopamine agonists, the unique pocket topology underlying ligand selectivity, the conformational changes in receptor activation, and potential structural determinants for G protein-coupling selectivity. These results provide both a molecular understanding of dopamine signaling and multiple structural templates for drug design targeting the dopaminergic system. |
| 资助项目 | National Key R&D Programs of China[2018YFA0507002] ; National Key R&D Programs of China[2019YFA0508800] ; Shanghai Municipal Science and Technology Major Projects[2019SHZDZX02] ; CAS Strategic Priority Research Program[XDB37030103] ; National Natural Science Foundation of China[81922071] ; National Natural Science Foundation of China[31770796] ; Zhejiang Province Natural Science Fund for Excellent Young Scholars[LR19H310001] ; Fundamental Research Funds for the Central Universities[2019XZZX001-01-06] ; Science and Technology Commission of Shanghai Municipality[20431900100] ; Jack Ma Foundation[2020-CMKYGG-05] ; National Science and Technology Major Projects[2018ZX09711002-002-002] ; NIMH (Psychoactive Drug Screening Program)[RO1MH112205] ; NIH[R35GM128641] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000629633400010 |
| 出版者 | CELL PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/295411]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Roth, Bryan L.; Zhang, Yan; Zhang, Cheng; Xu, H. Eric |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Biophys, Sch Med, Hangzhou 310058, Peoples R China 4.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Pathol, Sch Med, Hangzhou 310058, Peoples R China 5.Zhejiang Univ, Zhejiang Lab Syst & Precison Med, Med Ctr, Hangzhou 311121, Peoples R China 6.Zhejiang Univ, MOE Frontier Sci Ctr Brain Res & Brain Machine In, Sch Med, Hangzhou 310058, Peoples R China 7.Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15213 USA 8.Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA 9.Van Andel Res Inst, Ctr Canc & Cell Biol, Program Struct Biol, Grand Rapids, MI USA 10.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuang, Youwen,Xu, Peiyu,Mao, Chunyou,et al. Structural insights into the human D1 and D2 dopamine receptor signaling complexes[J]. CELL,2021,184(4):931-+. |
| APA | Zhuang, Youwen.,Xu, Peiyu.,Mao, Chunyou.,Wang, Lei.,Krumm, Brian.,...&Xu, H. Eric.(2021).Structural insights into the human D1 and D2 dopamine receptor signaling complexes.CELL,184(4),931-+. |
| MLA | Zhuang, Youwen,et al."Structural insights into the human D1 and D2 dopamine receptor signaling complexes".CELL 184.4(2021):931-+. |
入库方式： OAI收割
来源：上海药物研究所
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