Genome-Wide Mapping Reveals Complex Regulatory Activities of BfmR in Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Fan, Ke1,2; Cao, Qiao2; Lan, Lefu1,2,3,4
|
| 刊名 | MICROORGANISMS
 |
| 出版日期 | 2021-03-01 |
| 卷号 | 9期号:3页码:22 |
| 关键词 | Pseudomonas aeruginosa two-component system BfmRS regulon biofilm formation |
| DOI | 10.3390/microorganisms9030485 |
| 通讯作者 | Lan, Lefu(llan@simm.ac.cn) |
| 英文摘要 | BfmR is a response regulator that modulates diverse pathogenic phenotypes and induces an acute-to-chronic virulence switch in Pseudomonas aeruginosa, an important human pathogen causing serious nosocomial infections. However, the mechanisms of action of BfmR remain largely unknown. Here, using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we showed that 174 chromosomal regions of P. aeruginosa MPAO1 genome were highly enriched by coimmunoprecipitation with a C-terminal Flag-tagged BfmR. Integration of these data with global transcriptome analyses revealed that 172 genes in 106 predicted transcription units are potential targets for BfmR. We determined that BfmR binds to and modulates the promoter activity of genes encoding transcriptional regulators CzcR, ExsA, and PhoB. Intriguingly, BfmR bound to the promoters of a number of genes belong to either CzcR or PhoB regulon, or both, indicating that CzcRS and PhoBR two-component systems (TCSs) deeply feed into the BfmR-mediated regulatory network. In addition, we demonstrated that phoB is required for BfmR to promote the biofilm formation by P. aeruginosa. These results delineate the direct BfmR regulon and exemplify the complexity of BfmR-mediated regulation of cellular functions in P. aeruginosa. |
| 资助项目 | National Natural Science Foundation (NSFC)[31870127] ; National Natural Science Foundation (NSFC)[81861138047] ; Ministry of Science and Technology (MOST) of China[2016YFA0501503] ; Ministry of Science and Technology (MOST) of China[2019ZX09721001-004-003] ; Science and Technology Commission of Shanghai Municipality[19JC1416400] ; State Key Laboratory of Drug Research[SIMM2003ZZ-03] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000633897900001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/295419]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Lan, Lefu |
| 作者单位 | 1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.UCAS, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China 4.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Ke,Cao, Qiao,Lan, Lefu. Genome-Wide Mapping Reveals Complex Regulatory Activities of BfmR in Pseudomonas aeruginosa[J]. MICROORGANISMS,2021,9(3):22. |
| APA | Fan, Ke,Cao, Qiao,&Lan, Lefu.(2021).Genome-Wide Mapping Reveals Complex Regulatory Activities of BfmR in Pseudomonas aeruginosa.MICROORGANISMS,9(3),22. |
| MLA | Fan, Ke,et al."Genome-Wide Mapping Reveals Complex Regulatory Activities of BfmR in Pseudomonas aeruginosa".MICROORGANISMS 9.3(2021):22. |
入库方式： OAI收割
来源：上海药物研究所
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