中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2

文献类型：期刊论文

作者Hu, Shuwei1; Chen, Xiaoyu1,2; Zhu, Mengying1,2; Hu, Huifang1,2; Zhou, Chen1; Shi, Li1,2; Zhang, Naixia1,2
刊名PROTEIN EXPRESSION AND PURIFICATION
出版日期2021-05-01
卷号181页码:6
关键词Deubiquitinase USP20 DUSP domain NMR
ISSN号1046-5928
DOI10.1016/j.pep.2021.105836
通讯作者Shi, Li(shili@simm.ac.cn) ; Zhang, Naixia(nxzhang@simm.ac.cn)
英文摘要Deubiquitinase USP20/VDU2 (VHL-interacting Deubiquitinating Enzyme 2) has been proved to play vital roles in multiple cellular processes by controlling the life-span of substrate proteins including hypoxia-inducible factor HIF1 alpha, beta 2-adrenergic receptor, and type 2 iodothyronine deiodinase etc. USP20 contains four distinct structural domains, which include the N-terminal zinc-finger ubiquitin binding domain (ZnF-UBP), the catalytic domain (USP domain), and two tandem DUSP domains (DUSP1 and DUSP2). Here in this study, we report the setting up of the production approach for USP20 DUSP2, and the NMR characterization of the produced target protein. With the assistance of GB1 tag and glycerol, both the solubility and stability of USP20 DUSP2 are significantly enhanced. And by using the optimized protein production procedure, monomeric and stable N-15, C-13-labeled USP20 DUSP2 sample for NMR data acquisition was obtained. The secondary structural elements of USP20 DUSP2 were then revealed by the analysis of recorded NMR spectra, and USP20 DUSP2 forms an AB3 fold in solution. The production protocol and NMR characterization results reported in this manuscript could be utilized in the extended structural and functional studies of USP20 DUSP2.
资助项目National Natural Science Foundation of China[32000890] ; National Natural Science Foundation of China[21977105] ; National Natural Science Foundation of China[21778061]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000621214000007
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/295609]  
专题中国科学院上海药物研究所
通讯作者Shi, Li; Zhang, Naixia
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Shuwei,Chen, Xiaoyu,Zhu, Mengying,et al. Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2[J]. PROTEIN EXPRESSION AND PURIFICATION,2021,181:6.
APA Hu, Shuwei.,Chen, Xiaoyu.,Zhu, Mengying.,Hu, Huifang.,Zhou, Chen.,...&Zhang, Naixia.(2021).Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2.PROTEIN EXPRESSION AND PURIFICATION,181,6.
MLA Hu, Shuwei,et al."Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2".PROTEIN EXPRESSION AND PURIFICATION 181(2021):6.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。