中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa

文献类型：期刊论文

作者Xu, Chenchen1,2; Cao, Qiao2; Lan, Lefu1,2,3,4
刊名MICROORGANISMS
出版日期2021-02-01
卷号9期号:2页码:28
关键词Pseudomonas aeruginosa Glucose periplasmic binding protein two-component system transcriptional regulation
DOI10.3390/microorganisms9020447
通讯作者Lan, Lefu(llan@simm.ac.cn)
英文摘要A two-component system GtrS-GltR is required for glucose transport activity in P. aeruginosa and plays a key role during P. aeruginosa-host interactions. However, the mechanism of action of GtrS-GltR has not been definitively established. Here, we show that gltB, which encodes a periplasmic glucose binding protein, is essential for the glucose-induced activation of GtrS-GltR in P. aeruginosa. We determined that GltB is capable of binding to membrane regulatory proteins including GtrS, the sensor kinase of the GtrS-GltR TCS. We observed that alanine substitution of glucose-binding residues abolishes the ability of GltB to promote the activation of GtrS-GltR. Importantly, like the gtrS deletion mutant, gltB deletion mutant showed attenuated virulence in both Drosophila melanogaster and mouse models of infection. In addition, using CHIP-seq experiments, we showed that the promoter of gltB is the major in vivo target of GltR. Collectively, these data suggest that periplasmic binding protein GltB and GtrS-GltR TCS form a complex regulatory circuit that regulates the virulence of P. aeruginosa in response to glucose.
资助项目National Natural Science Foundation (NSFC)[31870127] ; National Natural Science Foundation (NSFC)[81861138047] ; Ministry of Science and Technology (MOST) of China[2016YFA0501503] ; Ministry of Science and Technology (MOST) of China[2019ZX09721001-004-003] ; Science and Technology Commission of Shanghai Municipality[19JC1416400] ; State Key Laboratory of Drug Research[SIMM2003ZZ-03]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000622823900001
出版者MDPI
源URL[http://119.78.100.183/handle/2S10ELR8/295655]  
专题新药研究国家重点实验室
通讯作者Lan, Lefu
作者单位1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.UCAS, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
4.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Chenchen,Cao, Qiao,Lan, Lefu. Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa[J]. MICROORGANISMS,2021,9(2):28.
APA Xu, Chenchen,Cao, Qiao,&Lan, Lefu.(2021).Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa.MICROORGANISMS,9(2),28.
MLA Xu, Chenchen,et al."Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa".MICROORGANISMS 9.2(2021):28.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。