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Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene

文献类型：期刊论文

作者Wang, Huinan2; Zhang, Libo2; Shang, Yuanbin2; Tan, Rongrong3; Ji, Mingxiang2; Yue, Xinliang2; Wang, Nannan2; Liu, Jun1; Wang, Chunhua2; Li, Yonggang4
刊名VIRUS EVOLUTION
出版日期2020-07-01
卷号6期号:2页码:10
关键词porcine epidemic diarrhea virus evolution recombination pathogenicity spike gene
DOI10.1093/ve/veaa049
通讯作者Li, Yonggang(lygjo@hotmail.com) ; Zhou, Tiezhong(ztz1818@126.com)
英文摘要Outbreaks of a new variant of porcine epidemic diarrhea virus (PEDV) at the end of 2010 have raised interest in the mutation and recombination of PEDV. A PEDV strain (CN/Liaoning25/2018) isolated from a clinical outbreak of piglet diarrhea contained a 49-bp deletion in the ORF3 gene. This deletion is considered a genetic characteristic of low pathogenic attenuated vaccine strains. However, CN/Liaoning25/2018 was highly pathogenic. Complete genome sequencing, identity analysis, phylogenetic tree construction, and recombination analysis showed that this virus was a recombinant strain containing the Spike (S) gene from the highly pathogenic CN/GDZQ/2014 strain and the remaining genomic regions from the low pathogenic vaccine isolate SQ2014. Histopathology and immunohistochemistry results confirmed that this strain was highly pathogenic and indicated that intestinal epithelial cell vacuolation was positively correlated with the intensity and density of PEDV antigens. A new natural recombination model for PEDV was identified. Our results suggest that new highly pathogenic recombinant strains in the field may be generated by recombination between low pathogenic attenuated live PEDV vaccines and pathogenic circulating PEDV strains. Our findings also highlight that the 49-bp deletion of the ORF3 gene in low pathogenic attenuated vaccine strains will no longer be a reliable standard to differentiate the classical vaccine attenuated from the field strains.
WOS关键词PCR ASSAY ; CORONAVIRUS ; DELTACORONAVIRUS ; DIVERSITY ; DIAGNOSIS ; AMERICAN ; GENOTYPE ; RECEPTOR ; OHIO
资助项目Liaoning Provincial Natural Science Foundation of China[2019-ZD-0804] ; Liaoning Provincial Natural Science Foundation of China[2019-ZD-0817] ; Liaoning Provincial Natural Science Foundation of China[2019-ZD-0836] ; Liaoning Provincial Natural Science Foundation of China[2019-ZD-0613] ; Liaoning Provincial Natural Science Foundation of China[2015020779] ; Jinzhou Medical University Undergraduate Training Programs for Innovation and Entrepreneurship[201827]
WOS研究方向Virology
语种英语
出版者OXFORD UNIV PRESS
WOS记录号WOS:000610104700012
源URL[http://119.78.100.183/handle/2S10ELR8/295936]  
专题中国科学院上海药物研究所
通讯作者Li, Yonggang; Zhou, Tiezhong
作者单位1.Beijing Institude Feed Conrrol, Beijing 100107, Peoples R China
2.Jinzhou Med Univ, Coll Anim Husb & Vet Med, Dept Basic Vet Med, Jinzhou 121000, Peoples R China
3.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
4.Jinzhou Med Univ, Sch Basic Med Sci, Dept Pathogen Biol, Jinzhou 121000, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Huinan,Zhang, Libo,Shang, Yuanbin,et al. Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene[J]. VIRUS EVOLUTION,2020,6(2):10.
APA Wang, Huinan.,Zhang, Libo.,Shang, Yuanbin.,Tan, Rongrong.,Ji, Mingxiang.,...&Zhou, Tiezhong.(2020).Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene.VIRUS EVOLUTION,6(2),10.
MLA Wang, Huinan,et al."Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene".VIRUS EVOLUTION 6.2(2020):10.

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来源：上海药物研究所

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