Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis
文献类型：期刊论文
| 作者 | Qian, Zhengming1,2; Wu, Zi2; Li, Chunhong2; Yao, ChangLiang3; Tan, Guoying2; Li, Wenjia2; Guo, De-an3
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| 刊名 | NATURAL PRODUCT COMMUNICATIONS
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| 出版日期 | 2021 |
| 卷号 | 16期号:1页码:7 |
| 关键词 | matrix solid-phase dispersion mannitol adenosine ergosterol medicinal mushrooms |
| ISSN号 | 1934-578X |
| DOI | 10.1177/1934578X20987784 |
| 通讯作者 | Li, Wenjia(liwenjia1111@163.com) ; Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | In this study, a multistep matrix solid-phase dispersion (MSPD) combining with a high-performance liquid chromatography methodwas developed for assaying 3 components of different polarities (mannitol, adenosine, and ergosterol) from mushroom samples. MSPD extraction was carried out using 1 g octadecyl-bonded silica as the sorbent material, 9% methanol, 20% methanol, and 100% methanol as eluting solvents for the elution of mannitol, adenosine, and ergosterol, respectively. Mannitol was separated on an NH2P-50 4E column and detected using an evaporative light scattering detector. Adenosine and ergosterol were separated on a Poroshell 120 SB-C18 column and measured at 260 nm and 283 nm, respectively. The developed method showed good linearity (R = 0.9986) within the test range. The relative SD (RSD) of precisions were less than 1.4%, and the recoveries were 95.6%-97.0% (RSD =3.0%). Compared with the reported methods, the developed procedure could rapidly prepare components with different polarities (mannitol, adenosine, and ergosterol) from medical mushroom samples with less organic solvent and sample. The method is rapid and eco-friendly, which is helpful to improve the quality evaluation of medicinal mushrooms. |
| WOS关键词 | PHOTODIODE-ARRAY ; CORE-SHELL ; EXTRACTION ; ERGOSTEROL ; ACID |
| 资助项目 | Key-Area Research and Development Program of Guangdong Province[2020B1111110007] ; Science and Technology Development Fund of Macao Special Administrative Region[125/2017/A3] |
| WOS研究方向 | Pharmacology & Pharmacy ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000608845500001 |
| 出版者 | SAGE PUBLICATIONS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/295960]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Wenjia; Guo, De-an |
| 作者单位 | 1.Xiangnan Univ, Dept Rehabil, Xiangnan, Hunan, Peoples R China 2.Dongguan HEC Cordyceps R&D Co Ltd, Key Lab State Adm Tradit Chinese Med, Dongguan 523850, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Zhengming,Wu, Zi,Li, Chunhong,et al. Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis[J]. NATURAL PRODUCT COMMUNICATIONS,2021,16(1):7. |
| APA | Qian, Zhengming.,Wu, Zi.,Li, Chunhong.,Yao, ChangLiang.,Tan, Guoying.,...&Guo, De-an.(2021).Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis.NATURAL PRODUCT COMMUNICATIONS,16(1),7. |
| MLA | Qian, Zhengming,et al."Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis".NATURAL PRODUCT COMMUNICATIONS 16.1(2021):7. |
入库方式： OAI收割
来源：上海药物研究所
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