中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography

文献类型：期刊论文

作者Jiang, Liyuan1,2,4,5; Cao, Yong1,2,4,5; Yin, Xianzhen6; Ni, Shuangfei1,2,4,5; Li, Miao1,2,4,5; Li, Chengjun1,2,4,5; Luo, Zixiang1,2,4,5; Lu, Hongbin1,3,4,5; Hu, Jianzhong1,2,4,5
刊名HISTOCHEMISTRY AND CELL BIOLOGY
出版日期2021-01-04
页码13
关键词SRμ CT Modified Golgi-Cox method Spinal cord Neuronal network Three-dimension
ISSN号0948-6143
DOI10.1007/s00418-020-01949-8
通讯作者Lu, Hongbin(hongbinlu@hotmail.com) ; Hu, Jianzhong(Jianzhonghu@hotmail.com)
英文摘要Exploring the three-dimensional (3D) morphology of neurons is essential to understanding spinal cord function and associated diseases comprehensively. However, 3D imaging of the neuronal network in the broad region of the spinal cord at cellular resolution remains a challenge in the field of neuroscience. In this study, to obtain high-resolution 3D imaging of a detailed neuronal network in the mass of the spinal cord, the combination of synchrotron radiation micro-computed tomography (SR mu CT) and the Golgi-cox staining were used. We optimized the Golgi-Cox method (GCM) and developed a modified GCM (M-GCM), which improved background staining, reduced the number of artefacts, and diminished the impact of incomplete vasculature compared to the current GCM. Moreover, we achieved high-resolution 3D imaging of the detailed neuronal network in the spinal cord through the combination of SR mu CT and M-GCM. Our results showed that the M-GCM increased the contrast between the neuronal structure and its surrounding extracellular matrix. Compared to the GCM, the M-GCM also diminished the impact of the artefacts and incomplete vasculature on the 3D image. Additionally, the 3D neuronal architecture was successfully quantified using a combination of SR mu CT and M-GCM. The SR mu CT was shown to be a valuable non-destructive tool for 3D visualization of the neuronal network in the broad 3D region of the spinal cord. Such a combinatorial method will, therefore, transform the presentation of Golgi staining from 2 to 3D, providing significant improvements in the 3D rendering of the neuronal network.
WOS关键词X-RAY MICROTOMOGRAPHY ; SYSTEM ; TISSUE ; HISTOLOGY ; SAMPLES ; MODEL
资助项目National Natural Science Foundation of China[81371956 81874004] ; National Natural Science Foundation of China[81672174] ; National Natural Science Foundation of China[81730068] ; Hunan Provincial Department of Finance[(2018)82] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts254] ; Key R&D Program of the Hunan Provincial Science & Technology Department[2017SK2061]
WOS研究方向Cell Biology ; Microscopy
语种英语
WOS记录号WOS:000604876700005
出版者SPRINGER
源URL[http://119.78.100.183/handle/2S10ELR8/296054]  
专题中国科学院上海药物研究所
通讯作者Lu, Hongbin; Hu, Jianzhong
作者单位1.Key Lab Organ Injury Aging & Regenerat Med Hunan, Changsha 410008, Peoples R China
2.Cent South Univ, Xiangya Hosp, Dept Spine Surg, Xiangya Rd 87, Changsha 410008, Hunan, Peoples R China
3.Cent South Univ, Xiangya Hosp, Dept Sports Med, Xiangya Rd 87, Changsha 410008, Hunan, Peoples R China
4.Xiangya Hosp, Int Chinese Musculoskeletal Res Soc, Sports Med Res Ctr, Changsha, Peoples R China
5.Hunan Engn Res Ctr Sport & Hlth, Changsha, Peoples R China
6.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Liyuan,Cao, Yong,Yin, Xianzhen,et al. A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2021:13.
APA Jiang, Liyuan.,Cao, Yong.,Yin, Xianzhen.,Ni, Shuangfei.,Li, Miao.,...&Hu, Jianzhong.(2021).A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography.HISTOCHEMISTRY AND CELL BIOLOGY,13.
MLA Jiang, Liyuan,et al."A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography".HISTOCHEMISTRY AND CELL BIOLOGY (2021):13.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。