Global and Site-Specific Effect of Phosphorylation on Protein Turnover
文献类型：期刊论文
| 作者 | Wu, Chongde3; Ba, Qian3; Lu, Dayun4,5; Li, Wenxue3; Salovska, Barbora3,8; Hou, Pingfu3; Mueller, Torsten6; Rosenberger, George2; Gao, Erli3; Di, Yi3 |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2021-01-11 |
| 卷号 | 56期号:1页码:111-+ |
| ISSN号 | 1534-5807 |
| DOI | 10.1016/j.devcel.2020.10.025 |
| 通讯作者 | Liu, Yansheng(yansheng.liu@yale.edu) |
| 英文摘要 | To date, the effects of specific modification types and sites on protein lifetime have not been systematically illustrated. Here, we describe a proteomic method, DeltaSILAC, to quantitatively assess the impact of site-specific phosphorylation on the turnover of thousands of proteins in live cells. Based on the accurate and reproducible mass spectrometry-based method, a pulse labeling approach using stable isotope-labeled amino acids in cells (pSILAC), phosphoproteomics, and a unique peptide-level matching strategy, our DeltaSILAC profiling revealed a global, unexpected delaying effect of many phosphosites on protein turnover. We further found that phosphorylated sites accelerating protein turnover are functionally selected for cell fitness, enriched in Cyclin-dependent kinase substrates, and evolutionarily conserved, whereas the glutamic acids surrounding phosphosites significantly delay protein turnover. Our method represents a generalizable approach and provides a rich resource for prioritizing the effects of phosphorylation sites on protein lifetime in the context of cell signaling and disease biology. |
| WOS关键词 | DATA-INDEPENDENT ACQUISITION ; CELL-CULTURE ; DYNAMICS ; QUANTIFICATION ; IDENTIFICATION ; PROTEOMICS ; STABILITY ; REVEALS ; PEPTIDE ; SILAC |
| 资助项目 | National Institute of General Medical Sciences (NIGMS) ; National Institutes of Health (NIH)[R01GM137031] ; Cancer Systems Biology@Yale ; Yale Cancer Center at Yale University ; Czech Academy of Sciences, Czech Republic[L200521953] ; Schram Stiftung[T0287/35359/2020] ; DFG[FO 1342/1-3] |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000607158400011 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296140]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Yansheng |
| 作者单位 | 1.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA 2.Columbia Univ, Dept Syst Biol, New York, NY USA 3.Yale Univ, Yale Canc Biol Inst, West Haven, CT 06516 USA 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 6.DKFZ, German Canc Res Ctr, D-69120 Heidelberg, Germany 7.Univ Med Ctr Gottingen, Dept Neuro & Sensory Physiol, D-37073 Gottingen, Germany 8.Czech Acad Sci, Dept Genome Integr, Inst Mol Genet, Prague, Czech Republic |
| 推荐引用方式 GB/T 7714 | Wu, Chongde,Ba, Qian,Lu, Dayun,et al. Global and Site-Specific Effect of Phosphorylation on Protein Turnover[J]. DEVELOPMENTAL CELL,2021,56(1):111-+. |
| APA | Wu, Chongde.,Ba, Qian.,Lu, Dayun.,Li, Wenxue.,Salovska, Barbora.,...&Liu, Yansheng.(2021).Global and Site-Specific Effect of Phosphorylation on Protein Turnover.DEVELOPMENTAL CELL,56(1),111-+. |
| MLA | Wu, Chongde,et al."Global and Site-Specific Effect of Phosphorylation on Protein Turnover".DEVELOPMENTAL CELL 56.1(2021):111-+. |
入库方式： OAI收割
来源：上海药物研究所
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